FlyBase Allele Report: Dmel\eRF1[U3]

General Information

Symbol

Dmel\eRF1^U3

Species

D. melanogaster

Name

FlyBase ID

FBal0102522

Feature type

allele

Associated gene

Dmel\eRF1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lukinova et al., 1999)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. Mutation is in the 3' splice site of intron 5, which is predicted to cause readthrough into intron 5, adding 28 nonspecific amino acids after E362 before a stop codon in exon 6.

(Chao et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:20,351,582..20,351,582 [+]

Nucleotide change:

G20351582A

Reported nucleotide change:

G?A

Comment:

G to A point mutation in the 3' splice site of the intron causes readthrough of the intron and early translation termination in the subsequent exon.

(Chao et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Chao et al., 2003, Lukinova et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

May be an antimorph.

(Chao et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

eRF1^U3/eRF1[+] is an enhancer of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

eRF1^U3/eRF1[+] is an enhancer of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Suppressor of

Statement

Reference

eRF1^U3 is a suppressor of phenotype of wg^PE4

(Chao et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

One copy of eRF1^U3 enhances the rough eye phenotype seen in flies expressing Hsap\MAPT^GMR.Ex.PJ. Necrotic plaques are also seen.

(Ambegaokar and Jackson, 2011)

Complementation and Rescue Data

Fails to complement

eRF1²⁶⁰⁴

(Collins and Cohen, 2005)

eRF1²⁶³³

(Collins and Cohen, 2005)

eRF1^KY7

(Chao et al., 2003)

eRF1^A7

(Chao et al., 2003)

eRF1^C1

(Chao et al., 2003)

eRF1^C2

(Chao et al., 2003)

eRF1^F2

(Chao et al., 2003)

eRF1^K3

(Chao et al., 2003)

eRF1^K7

(Chao et al., 2003)

eRF1^V2

(Chao et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5466

eRF1^U3/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

(Lukinova et al., 1999)

eRF1^U3

(Ambegaokar and Jackson, 2011)

l(3)77ABb^U3

l(3)neo28^U3

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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