FlyBase Allele Report: Dmel\hth[HM.UAS.GFP]

General Information

Symbol

Dmel\hth^HM.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0102683

Feature type

allele

Associated gene

Dmel\hth

Associated Insertion(s)

Carried in Construct

P{UAS-GFP-hth.HM}

Key Links

Transgenic product class

inframe_deletion

(Ryoo et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Ryoo et al., 1999)

Mutagen

in vitro construct

(Ryoo et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-GFP-hth.HM}

Cytology

Description

UASt regulatory sequences drive expression of amino acids 36 to 245 of hth tagged with GFP.

(Ryoo et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

hth

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Dll-md23

(Stevens and Mann, 2007, Ryoo et al., 1999)

visible | homeotic, with Scer\GAL4^hth-GETDB

(Noro et al., 2006)

visible | homeotic | male, with Scer\GAL4^Dll-md23

(Ryoo et al., 1999)

visible | homeotic | male, with Scer\GAL4^pnr-MD237

(Ryoo et al., 1999)

Phenotype Manifest In

abdominal tergite | male, with Scer\GAL4^pnr-MD237

(Ryoo et al., 1999)

antenna, with Scer\GAL4^hth-GETDB

(Noro et al., 2006)

antenna | distal, with Scer\GAL4^Dll-md23

(Ryoo et al., 1999)

dorsal margin photoreceptor cell R7, with Scer\GAL4^GMR.long

(Wernet et al., 2003)

dorsal margin photoreceptor cell R8, with Scer\GAL4^GMR.long

(Wernet et al., 2003)

leg, with Scer\GAL4^Dll-md23

(Ryoo et al., 1999)

leg | distal, with Scer\GAL4^Dll-md23

(Stevens and Mann, 2007)

leg | ectopic, with Scer\GAL4^hth-GETDB

(Noro et al., 2006)

photoreceptor cell R8 | ectopic, with Scer\GAL4^GMR.long

(Wernet et al., 2003)

Detailed Description

Statement

Reference

Expression of hth^{HM.Scer\UAS.T:Avic\GFP} in all photoreceptor neurons under the control of Scer\GAL4^GMR.PF alters the dual terminals seen in the dorsal-posterior M6 medulla to single terminals.

(Chin et al., 2014)

Expression of hth^{HM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Dll-md23 disrupts distal leg patterning.

(Stevens and Mann, 2007)

Expression of hth^{HM.Scer\UAS.T:Avic\GFP}, under the control of Scer\GAL4^hth-GETDB causes an antenna-to-leg transformation in 69% of cases. Scer\GAL4^hth-GETDB>hth^{HM.Scer\UAS.T:Avic\GFP} flies rarely show loss of wing blades and halteres.

Flies coexpressing hth^{Scer\UAS.T:Avic\GFP} and hth^{HM.Scer\UAS.T:Avic\GFP}, both under the control of Scer\GAL4^hth-GETDB, show a significant reversion of the phenotypes observed when each is expressed individually.

(Noro et al., 2006)

Adults expressing hth^{HM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.long have no obvious external eye phenotype. In the dorsal rim area (DRA) of the eye, the R8 photoreceptor cells have lost their DRA identity and appear to have chosen the regular R8 cell fate. The nuclei of DRA R8 cells are located significantly more basally than normal, as is typical for the regular R8 cells found throughout the rest of the eye. All central photoreceptor cells (R7 and R8) in the DRA have lost their typical large rhabdomere in these flies.

(Wernet et al., 2003)

Expression of hth^{HM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^dpp.blk1 or Scer\GAL4^ptc-559.1 does not suppress eye development. Expression of hth^{HM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ptc-559.1 does not produce a split wing phenotype. Expression of hth^{HM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Dll-md23 results in leg truncations and the transformation of distal antenna into distal leg. Expression of hth^{HM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^pnr-MD237 results in an increase in pigmentation in anterior tergites of males, consistent with an anterior to posterior transformation of abdominal segment identity. No effect on trichome density is seen in these flies, suggesting the transformation is incomplete.

(Ryoo et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hth^{HM.Scer\UAS.T:Avic\GFP}

hth^HM.UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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