FlyBase Allele Report: Dmel\how[L.UAS]

General Information

Symbol

Dmel\how^L.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0102691

Feature type

allele

Associated gene

Dmel\how

Associated Insertion(s)

Carried in Construct

P{UAS-how.L}

Also Known As

UAS-How(L)

Key Links

Transgenic product class

cDNA

(Nabel-Rosen et al., 1999)

wild_type

(Nabel-Rosen et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Nabel-Rosen et al., 1999)

wild_type

(Nabel-Rosen et al., 1999)

Mutagen

in vitro construct

(Nabel-Rosen et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-how.L}

Cytology

Description

The how L cDNA is expressed under the control of UASt regulatory sequences.

(Nabel-Rosen et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

how

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number, with Scer\GAL4^{VP16.nanos.UTR}

(Monk et al., 2010)

Phenotype Manifest In

spermatogonial cyst, with Scer\GAL4^{VP16.nanos.UTR}

(Monk et al., 2010)

spermatogonium, with Scer\GAL4^{VP16.nanos.UTR}

(Monk et al., 2010)

testis, with Scer\GAL4^{VP16.nanos.UTR}

(Monk et al., 2010)

Detailed Description

Statement

Reference

Overexpression of how^L.Scer\UAS in the germline via Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in testes larger in size than in wild-type, with the most notable difference being an expansion of the apex of the testis. Overexpression of how^L.Scer\UAS causes a hyper-proliferation of spermatogonial cells.

In wild-type testes, the maximum number of spermatogonia within a cyst is 16, which differentiate into 16 spermatocytes. In Scer\GAL4^{nos.UTR.T:Hsim\VP16}->how^L.Scer\UAS testes, cysts of 32 and 64 spermatocytes are frequently observed. This failure to exit mitotic division is not permanent, as germ cell tumours are not apparent, and spermatogonial cysts with increased numbers can differentiate into spermatocytes. Cysts containing 32 spermatocytes are able to undergo meiosis correctly; cysts of 128 early spermatids are observed, which is double the number found in wild-type.

Expression of how^L.Scer\UAS under the control of Scer\GAL4^nos.PG has little effect on germline stem cell development.

(Monk et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^nanos.PG/how^L.UAS is an enhancer of abnormal cell cycle phenotype of bam^Δ86

(Monk et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^nanos.PG/how^L.UAS is an enhancer of spermatocyte cyst phenotype of bam^Δ86

(Monk et al., 2010)

Scer\GAL4^nanos.PG/how^L.UAS is an enhancer of spermatocyte phenotype of bam^Δ86

(Monk et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of how^L.Scer\UAS under the control of the weak driver Scer\GAL4^nos.PG enhances the bam^Δ86/+ phenotype, as shown by the fact that 65% of cysts containing 32 spermatocytes, compared to 27% in bam^Δ86/+ single mutants.

(Monk et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{VP16.nanos.UTR}/how^L.UAS rescues how^r17/how^r4

(Monk et al., 2010)

Comments

Expression of how^L.Scer\UAS in early germline stem cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the how^r17/how^r4 germline stem cell phenotype.

(Monk et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

how^L.Scer\UAS

how^L.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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