FlyBase Allele Report: Dmel\how[r4]

General Information

Symbol

Dmel\how^r4

Species

D. melanogaster

Name

FlyBase ID

FBal0060393

Feature type

allele

Associated gene

Dmel\how

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Baehrecke, 1997)

Mutagen

P-element activity

(Baehrecke, 1997)

Progenitor genotype

P{lacW}B1-93F

(Baehrecke, 1997)

Caused by aberration

Df(3R)who^r4

Cytology

Description

Imprecise excision of the P-element.

(Baehrecke, 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage (with how^r17)

(Monk et al., 2010)

some die during pupal stage (with how^r17)

(Monk et al., 2010)

Phenotype Manifest In

germline cyst | germline clone (with how^r17)

(Monk et al., 2010)

male germline stem cell | germline clone (with how^r17)

(Monk et al., 2010)

testis | germline clone (with how^r17)

(Monk et al., 2010)

Detailed Description

Statement

Reference

Approximately 36% of how^r17/how^r4 mutant testes completely lack germline stem cells. Another 16% of testes show significant gaps in the ring of vas-positive cells, indicating a reduction in the number of germline stem cells. The remaining mutant germ cells are not found in organized cysts and different-sized germ cells are observed near the hub.

(Monk et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

how^r17/how^r4 is rescued by Scer\GAL4^{VP16.nanos.UTR}/how^L.UAS

(Monk et al., 2010)

how^r17/how^r4 is rescued by Scer\GAL4^{VP16.nanos.UTR}/how^S.UAS

(Monk et al., 2010)

Comments

Expression of how^S.Scer\UAS in early germline stem cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the how^r17/how^r4 germline stem cell phenotype.

Expression of how^L.Scer\UAS in early germline stem cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the how^r17/how^r4 germline stem cell phenotype.

(Monk et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

how^r4

(Monk et al., 2010)

who^r4

(Baehrecke, 1997)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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