FlyBase Allele Report: Dmel\Klp64D[k5]

General Information

Symbol

Dmel\Klp64D^k5

Species

D. melanogaster

Name

FlyBase ID

FBal0102963

Feature type

allele

Associated gene

Dmel\Klp64D

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Ray et al., 1999)

Progenitor genotype

Cytology

Description

Amino acid replacement: E551K.

(Ray et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:5,357,604..5,357,604 [-]

Nucleotide change:

G5357604A

Reported nucleotide change:

G?A

Amino acid change:

E551K | Klp64D-PA

Reported amino acid change:

E551K

(Ray et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception (with Klp64D^k1)

(Sarpal et al., 2003)

abnormal auditory perception (with Klp64D^l4)

(Sarpal et al., 2003)

abnormal auditory perception | recessive

(Sarpal et al., 2003)

abnormal neuroanatomy

(Jana et al., 2011)

abnormal neuroanatomy (with Klp64D^k1)

(Jana et al., 2011)

abnormal neuroanatomy (with Klp64D^l4)

(Jana et al., 2011)

abnormal neurophysiology (with Klp64D^k1)

(Jana et al., 2011, Sarpal et al., 2003)

abnormal neurophysiology (with Klp64D^l4)

(Jana et al., 2011, Sarpal et al., 2003)

abnormal neurophysiology | recessive

(Jana et al., 2011, Sarpal et al., 2003)

decreased rate of movement | recessive

(Ray et al., 1999)

lethal | recessive

(Ray et al., 1999)

uncoordinated | adult stage | recessive

(Ray et al., 1999)

Phenotype Manifest In

basiconic sensillum of antennal segment 3

(Jana et al., 2011)

basiconic sensillum of antennal segment 3 (with Klp64D^k1)

(Jana et al., 2011)

basiconic sensillum of antennal segment 3 (with Klp64D^l4)

(Jana et al., 2011)

cilium

(Jana et al., 2011)

cilium (with Klp64D^k1)

(Jana et al., 2011)

cilium (with Klp64D^l4)

(Jana et al., 2011)

Johnston's organ & scolopidial cilium

(Sarpal et al., 2003)

Johnston organ (with Klp64D^l4)

(Sarpal et al., 2003)

scolopidial cilium & Johnston's organ (with Klp64D^l4)

(Sarpal et al., 2003)

Detailed Description

Statement

Reference

The average peak electroantennogram response is reduced in homozygous, Klp64D^k5/Klp64D^l4 and Klp64D^k5/Klp64D^k1 adults compared to controls (in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid).

The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in mutant adults, but the outer segments of the basiconic cilia are less branched than in wild type.

The electron-dense basal bodies and the connecting cilium are often present in the sensilla basiconica of the antenna in Klp64D^k5/Klp64D^l4 adults, but the outer segments of the basiconic cilia usually contain a slender extension with several singlet microtubules.

The outer segments of the basiconic cilia of the sensilla basiconica of the antenna are less branched than normal in Klp64D^k5/Klp64D^k1 adults.

(Jana et al., 2011)

Klp64D^k5 homozygotes, Klp64D^k1/Klp64D^k5, and Klp64D^l4/Klp64D^k5 animals have a reduced auditory response, producing a severely reduced sound evoked potentials from the Johnston's organ neurons. The Johnston organ scolopidia contains complete sets of cilia. The basal body structures, ciliary roots and desmosomal junctions between inner dendritic segments appear normal. However, the ciliary dilations are deformed and disorganised and are often located in the same plane as the dendritic caps.

(Sarpal et al., 2003)

Homozygotes die at or before the early third larval instar stage in a typical crowded culture. If the homozygotes are raised in low density conditions without competition from wild-type larvae, a few mutant animals survive to be pharate pupae and occasionally eclose as fully formed adults. The adults show an acutely uncoordinated walk, are unable to stand and die in a few hours. Mutant third instar larvae show slight to severe sluggishness and roll abnormally from side to side during crawling.

(Ray et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Klp64D^k5/Klp64D^l4 has abnormal auditory perception phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Klp64D^k5/Klp64D^l4 has abnormal neurophysiology phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Suppressor of

Statement

Reference

Klp64D^k5 is a suppressor of paralytic | posterior | larval stage phenotype of Aplip1^EK4

(Horiuchi et al., 2005)

Other

Statement

Reference

Kap3^V6, Klp64D^k5/Klp64D^l4 has lethal phenotype

(Sarpal et al., 2003)

Kap3^V6, Klp64D^k5/Klp64D^l4 has partially lethal phenotype

(Sarpal et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Klp64D^k5/Klp64D^l4 has Johnston organ phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Klp64D^k5/Klp64D^l4 has scolopidial cilium & Johnston's organ phenotype, enhanceable by Kap3^V6

(Sarpal et al., 2003)

Suppressor of

Statement

Reference

Klp64D^k5 is a suppressor of axon phenotype of Aplip1^EK4

(Horiuchi et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

In Aplip1^EK4, Klp64D^k5 double mutants the posterior paralysis and axonal swelling of Aplip1^EK4 single mutants are suppressed.

(Horiuchi et al., 2005)

The addition of Kap3^V6/+ significantly enhances the recessive lethality of Klp64D^l4/Klp64D^k5 animals. The addition of Kap3^V6/Y causes complete lethality. The addition of Kap3^V6/+ to Klp64D^l4/Klp64D^k5 also completely abolishes the sound evoked potentials usually obtained from the Johnston's organ neurons, and enhances the ciliary defects seen in these animals.

(Sarpal et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Klp64Dⁿ¹²³

(Ray et al., 1999)

Rescued by

Klp64D^k5/Klp64D^k1 is rescued by Scer\GAL4^da.PU/Klp64D^UAS.cRa

(Jana et al., 2011)

Klp64D^k5 is rescued by Scer\GAL4^JO15/Klp64D^UAS.cRa

(Sarpal et al., 2003)

Klp64D^k5 is rescued by Scer\GAL4^Tm1-C817/Klp64D^UAS.cRa

(Sarpal et al., 2003)

Klp64D^k5 is rescued by Scer\GAL4^MJ94/Klp64D^UAS.cRa

(Sarpal et al., 2003)

Klp64D^k5/Klp64Dⁿ¹²³ is rescued by Klp64D^UAS.cRa

(Ray et al., 1999)

Klp64D^k5 is rescued by Klp64D^UAS.cRa

(Ray et al., 1999)

Comments

Expression of Klp64D^Scer\UAS.cRa under the control of Scer\GAL4^da.G32 rescues the reduced electroantennogram response of Klp64D^k5/Klp64D^k1 adults in response to ethyl acetate, n-butanol, benzaldehyde or propionic acid.

(Jana et al., 2011)

Klp64D^Scer\UAS.cRa rescues the lethality and uncoordinated behaviour of Klp64D^k5, either in homozygous condition or in transheterozygous combination with Klp64Dⁿ¹²³, in the absence of a Scer\GAL4 driver.

(Ray et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Klp64D^k5

(Dey and Ray, 2018, Jana et al., 2011, Mattie et al., 2010, Baqri et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (7)

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