FlyBase Allele Report: Dmel\Kap3[V6]

General Information

Symbol

Dmel\Kap3^V6

Species

D. melanogaster

Name

FlyBase ID

FBal0156305

Feature type

allele

Associated gene

Dmel\Kap3

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Sarpal et al., 2003)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception | recessive

(Sarpal et al., 2003)

abnormal neuroanatomy

(Jana et al., 2011)

abnormal neurophysiology

(Jana et al., 2011)

abnormal neurophysiology | recessive

(Sarpal et al., 2003)

lethal - all die before end of pupal stage | recessive

(Sarpal et al., 2003)

some die during pupal stage | recessive

(Sarpal et al., 2003)

uncoordinated | recessive

(Sarpal et al., 2003)

Phenotype Manifest In

basiconic sensillum of antennal segment 3

(Jana et al., 2011)

cilium

(Jana et al., 2011)

Johnston's organ & scolopidial cilium

(Sarpal et al., 2003)

labellar taste bristle

(Jana et al., 2011)

trichoid sensillum of antennal segment 3

(Jana et al., 2011)

Detailed Description

Statement

Reference

Mutant adults show no electroantennogram response to a number of odorants.

Cilia are absent on nearly 80% of olfactory receptor neurons of the sensilla basiconica and sensilla trichodea of the antenna in mutant animals. The gustatory cilia inside labellar hairs are also absent.

(Jana et al., 2011)

Occasional escapers are seen that emerge as uncoordinated adults. Mutant pupae have neuronal cell bodies that appear to lack their cilia - mutant animals have deformities in the ciliary substructure. cilia are almost completely missing, sections at the midscolopale level show a lack of axonemal profiles, though basal bodies and roots are visible in many scolopales. Occasionally scolpales are seen with thin deformed membranous cilia extending from a distal body. Both homozygotes and Kap3^V6/Df(1)RA37 completely lose their ability to produce sound evoked potentials from the Johnston's organ neurons. Electroretinogram responses are normal. Sperm axoneme growth is unaffected in homozygotes.

(Sarpal et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Kap3^V6 has abnormal neurophysiology phenotype, suppressible by Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Kap3^V6 has abnormal neuroanatomy phenotype, suppressible by Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

NOT suppressed by

Statement

Reference

Kap3^V6 has abnormal neurophysiology phenotype, non-suppressible by Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Enhancer of

Statement

Reference

Kap3^V6 is an enhancer of abnormal auditory perception phenotype of Klp64D^k5/Klp64D^l4

(Sarpal et al., 2003)

Kap3^V6 is an enhancer of abnormal neurophysiology phenotype of Klp64D^k5/Klp64D^l4

(Sarpal et al., 2003)

Other

Statement

Reference

Kap3^V6, Klp64D^k5/Klp64D^l4 has lethal phenotype

(Sarpal et al., 2003)

Kap3^V6, Klp64D^k5/Klp64D^l4 has partially lethal phenotype

(Sarpal et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

Kap3^V6 has trichoid sensillum of antennal segment 3 phenotype, suppressible by Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Kap3^V6 has basiconic sensillum of antennal segment 3 phenotype, suppressible by Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Kap3^V6 has cilium phenotype, suppressible by Klp64D^UAS.GFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

NOT suppressed by

Statement

Reference

Kap3^V6 has trichoid sensillum of antennal segment 3 phenotype, non-suppressible by Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Kap3^V6 has basiconic sensillum of antennal segment 3 phenotype, non-suppressible by Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Kap3^V6 has cilium phenotype, non-suppressible by Klp68D^UAS.YFP/Scer\GAL4^ChAT.7.4

(Jana et al., 2011)

Enhancer of

Statement

Reference

Kap3^V6 is an enhancer of Johnston organ phenotype of Klp64D^k5/Klp64D^l4

(Sarpal et al., 2003)

Kap3^V6 is an enhancer of scolopidial cilium & Johnston's organ phenotype of Klp64D^k5/Klp64D^l4

(Sarpal et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Klp64D^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Cha.7.4 partially restores cilia growth in the sensilla basiconica and sensilla trichodea of the antennae in Kap3^V6 adults (cilia length is restored almost to wild type, while cilia volume is restored to approximately 25% of the normal level). Odor-evoked electroantennogram responses are also partly restored in these animals (assayed in response to ethyl acetate, n-butanol, benzaldehyde and propionic acid).

Expression of Klp68D^{Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^Cha.7.4 does not rescue cilia growth in the sensilla basiconica and sensilla trichodea of the antennae or odor-evoked electroantennogram responses in Kap3^V6 adults.

(Jana et al., 2011)

The addition of Kap3^V6/+ significantly enhances the recessive lethality of Klp64D^l4/Klp64D^k5 animals. The addition of Kap3^V6/Y causes complete lethality. The addition of Kap3^V6/+ to Klp64D^l4/Klp64D^k5 also completely abolishes the sound evoked potentials usually obtained from the Johnston's organ neurons, and enhances the ciliary defects seen in these animals.

(Sarpal et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Kap3^V6 is rescued by Scer\GAL4^SG18.1/Kap3^UAS.cSa

(Jana et al., 2011)

Kap3^V6 is rescued by Kap3^+t12

(Jana et al., 2011)

Kap3^V6 is rescued by Kap3^+t20

(Jana et al., 2011)

Kap3^V5/Kap3^V6 is rescued by Scer\GAL4^SG18.1/Kap3^UAS.cSa

(Sarpal et al., 2003)

Kap3^V5/Kap3^V6 is rescued by Scer\GAL4^elav-C155/Kap3^UAS.cSa

(Sarpal et al., 2003)

Kap3^V6 is rescued by Scer\GAL4^JO15/Kap3^UAS.cSa

(Sarpal et al., 2003)

Kap3^V6 is rescued by Scer\GAL4^elav-C155/Kap3^UAS.cSa

(Sarpal et al., 2003)

Comments

Kap3^+t12 and Kap3^+t20 rescue the odor-evoked electroantennogram response in Kap3^V6 animals (assayed in response to ethyl acetate, n-butanol, benzaldehyde and propionic acid).

Expression of Kap3^Scer\UAS.cSa under the control of Scer\GAL4^SG18.1 rescues the odor-evoked electroantennogram response in Kap3^V6 animals (assayed in response to ethyl acetate, n-butanol, benzaldehyde and propionic acid).

(Jana et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DmKap3^V6

(Sarpal et al., 2003)

DmKap^v6

(Jana et al., 2011)

Kap3^V6

Name Synonyms

Secondary FlyBase IDs

References (2)

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