UASt regulatory sequences drive expression of shg carrying amino acid replacements G1010L, S1011L and P1012L. The coding sequence is tagged at the C-terminal end with sgGFP.
Scer\GAL4btl.PS/shgmNcGSP.UAS.sgGFP rescues Df(2R)E2/shg2
The addition of shgmNcGSP.Scer\UAS.T:Avic\GFP-rs, driven by Scer\GAL4btl.PS, into shg2/Df(2R)E2 flies rescues their tracheal fusion phenotype.