UASt regulatory sequences drive expression of shg in which amino acids YPYDVPDYA (a Tag:HA epitope) have been inserted between amino acids 979 and 980. The coding sequence is tagged at the C-terminal end with sgGFP.
shginNcHA.UAS.sgGFP/Scer\GAL4btl.PS fails to rescue Df(2R)E2/shg2
The addition of shginNcHA.Scer\UAS.T:Avic\GFP-rs, driven by Scer\GAL4btl.PS, into shg2/Df(2R)E2 flies fails to rescue their tracheal fusion phenotype.