FlyBase Allele Report: Dmel\shg[dNc.UAS.sgGFP]

General Information

Symbol

Dmel\shg^{dNc.UAS.sgGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0103560

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{UAS-dNc}

Also Known As

dNc

Key Links

Transgenic product class

inframe_deletion

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

(Oda and Tsukita, 1999)

Progenitor genotype

Carried in construct

P{UAS-dNc}

Cytology

Description

UASt regulatory sequences drive expression of shg in which amino acids 939-1019 are deleted. The coding sequence is tagged at the C-terminal end with sgGFP.

(Oda and Tsukita, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

shg

Tagged with

sgGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^upd1.PU

(Lim et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

Phenotype Manifest In

cyst progenitor cell | adult stage, with Scer\GAL4^upd1.PU

(Lim et al., 2015)

cyst progenitor cell | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

hub cell | adult stage, with Scer\GAL4^upd1.PU

(Lim et al., 2015)

hub cell | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

male germline stem cell | adult stage, with Scer\GAL4^upd1.PU

(Lim et al., 2015)

male germline stem cell | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Lim et al., 2015)

Detailed Description

Statement

Reference

Expression of shg^{dNc.Scer\UAS.T:Avic\GFP-rs} under the control of either Scer\GAL4^{nos.UTR.T:Hsim\VP16} or Scer\GAL4^upd1.PU results in significantly decreased numbers of hub cells, germline stem cells and cyst stem cells in the testicular niche.

(Lim et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^btl.PS/shg^{dNc.UAS.sgGFP} fails to rescue Df(2R)E2/shg²

(Oda and Tsukita, 1999)

Comments

The addition of shg^{dNc.Scer\UAS.T:Avic\GFP-rs}, driven by Scer\GAL4^btl.PS, into shg²/Df(2R)E2 flies fails to rescue their tracheal fusion phenotype.

(Oda and Tsukita, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E-Cad^{unprocessable}

(Lim et al., 2015)

dNc

(Lim et al., 2015)

shg^{dNc.Scer\UAS.T:Avic\GFP-rs}

shg^{dNc.UAS.rsGFP}

shg^{dNc.UAS.sgGFP}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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