FlyBase Allele Report: Dmel\piwi[EP1024]

General Information

Symbol

Dmel\piwi^EP1024

Species

D. melanogaster

Name

FlyBase ID

FBal0103599

Feature type

allele

Associated gene

Dmel\piwi

Associated Insertion(s)

P{EP}piwi^EP1024

Carried in Construct

Also Known As

piwi^EP, ep(2)1024

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

wild-type allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Cox et al., 2000)

wild-type allele

(Cox et al., 2000)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Cox et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}piwi^EP1024

Cytology

Description

P{EP} insertion in the 5' untranslated region. The P{EP} element is oriented in the same direction as the direction of piwi transcription, such that an mRNA containing the complete piwi open reading frame is produced when expression is driven by the Scer\UAS regulatory sequences in the P{EP} element.

(Cox et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:10,987,380..10,987,380 [-]

(Hamada-Kawaguchi et al., 2014, Szakmary et al., 2005, Gene Disruption Project members, 2001-, Cox et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | oogenesis, with Scer\GAL4^hs.PB

(Cox et al., 2000)

Phenotype Manifest In

female germline stem cell | ectopic, with Scer\GAL4^hs.PB

(King et al., 2001, Cox et al., 2000)

female germline stem cell | increased number | heat sensitive, with Scer\GAL4^hs.PB

(Yang et al., 2007)

germarium, with Scer\GAL4^hs.PB

(Cox et al., 2000)

germline cell | female, with Scer\GAL4^hs.PB

(Cox et al., 2000)

nurse cell spectrosome, with Scer\GAL4^hs.PB

(King et al., 2001)

ovariole (with piwi¹)

(Szakmary et al., 2005)

ovary (with piwi¹)

(Cox et al., 2000)

spectrosome, with Scer\GAL4^hs.PB

(Cox et al., 2000)

spectrosome | ectopic, with Scer\GAL4^hs.PB

(Cox et al., 2000)

testis (with piwi¹)

(Cox et al., 2000)

Detailed Description

Statement

Reference

Expression of piwi^EP1024 under the control of Scer\GAL4^hs.PB in females using heat shock results in an increase in the number of spectrosome-containing germ cells per germarium compared to wild-type controls.

(Yang et al., 2007)

Females with piwi^EP1024, driven by Scer\GAL4^hs.PB (and heatshocked) have germaria with an increased number (Three times that of wild-type) of spectrosome containing germ-line cells. The numbers of stalk cells is unaffected.

(King et al., 2001)

piwi^EP1024/piwi¹ ovaries and testes show a typical piwi¹ mutant phenotype. Overexpression of piwi^EP1024 under the control of Scer\GAL4^hs.PB using heat shock in a wild-type background increases the number of spectrosome-containing germ cells to an average of 7.5 cells per germarium (compared to 2-3 cells per wild-type germarium). In the most extreme cases, up to 15 spectrosome-containing cells are found in a single germarium. The extra spectrosome-containing cells appear to be germline stem cell (GSC)-like cells. They incorporate BrdU at a level similar to wild-type GSCs. These GSC-like cells can all differentiate within 4 days following the withdrawal of heat shock, leaving the germaria with only 2-3 GSCs at their normal position. No signs of cell death, such as pycnotic nuclei or apoptotic bodies are seen, suggesting they are capable of oogenesis. The extra GSC-like cells induced by overexpression of piwi^EP1024 under the control of Scer\GAL4^hs.PB using heat shock show an even distribution pattern along the germarial axis in both regions 1 and 2a (in wild-type germaria, spectrosome-containing cells are exclusively located in the apical area of region 1). Spectrosome-containing cells are not seen in regions 2b and 3 of the germarium, instead 16-cell cysts develop normally to form egg chambers. In germaria in which piwi^EP1024 is overexpressed under the control of Scer\GAL4^hs.PB using heat shock, 31% of germaria contain a single GSC with a telophase spectrosome (compared to 20-25% in wild type) and 13% of germaria contain two GSCs with telophase spectrosomes (compared to 2-3% in wild type). 3% of germaria contain three GSCs with telophase spectrosomes (not seen in wild-type germaria).

(Cox et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

piwi^EP1024/piwi¹ is rescued by Scer\GAL4^hs.PB, piwi^EP1024

(Cox et al., 2000)

Rescues

Scer\GAL4^hs.PB, piwi^EP1024 rescues piwi^EP1024/piwi¹

(Cox et al., 2000)

Comments

When piwi^EP1024 is overexpressed under the control of Scer\GAL4^hs.PB using heat shock treatment (1 hour at 37^oC every day starting from the larval stage) in a piwi^EP1024/piwi¹ background, significant rescue of the piwi^EP1024/piwi¹ mutant phenotype is seen in both females and males. Specifically, in females, germline stem cells are maintained and a string of developing egg chambers are observed, while in males, germline stem cells are also rescued and spermatogenesis is fully restored. Fertility is rescued to wild-type levels. Heat shock starting at the pupal stage does not rescue fertility.

(Cox et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ep(2)1024

(King et al., 2001, Cox et al., 2000)

piwi^EP-1024

(Chen and McKearin, 2005)

piwi^EP1024

piwi^EP

(Yang et al., 2007, Chen and McKearin, 2005, Szakmary et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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