Homozygous clones at the edge of the eye do not result in ectopic photoreceptors.

Mutant embryos derived from females containing homozygous spen³ germline clones crossed to spen⁵/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. The number of lateral chordotonal organs in each abdominal hemisegment varies from 0 to 6, and is typically 4 (wild-type number is 5). Clusters containing the normal number are often disorganised. Mutant embryos derived from females containing homozygous spen⁵ germline clones crossed to spen³/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. Mutant embryos derived from females containing homozygous spen^poc361 germline clones crossed to spen³/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. The number of lateral chordotonal organs in each abdominal hemisegment varies from 0 to 6, and is typically 4 (wild-type number is 5). Clusters containing the normal number are often disorganised. Midline development is defective. Commissures are missing or poorly separated in stage 15 embryos. All of the longitudinal axons are disrupted in stage 16 embryos and axons inappropriately cross the midline. The development of all motor axons pathways is defective; motor axons exit the central nervous system, pick the correct pathways, but fail to innervate their muscle targets. Muscle fibres are missing or disorganised. Mutant embryos derived from females containing homozygous spen^poc231 germline clones crossed to spen³/+ males (lacking both maternal and zygotic spen function) show alterations in the number of many peripheral and central nervous system cell types, and the development of other organs is affected. The number of lateral chordotonal (lch) organs in each abdominal hemisegment varies from 0 to 6, and is typically 4 (wild-type number is 5). Clusters containing the normal number are often disorganised. The lch axons stall prematurely. The intersegmental nerve motor axon pathway and commissural central nervous system axon tracts are normal in homozygous, hemizygous or spen³/spen⁵ embryos. Defects are seen in the elongation and pathfinding of axons in the intersegmental nerve b (ISNb) and segmental nerve a (SNa) motor axon pathways and in the transverse nerve.

spen[+]/spen³ is a suppressor of visible phenotype of Hipk^KK107857, Scer\GAL4^ey.PH

spen[+]/spen³ is a suppressor of abnormal size phenotype of Hipk^KK107857, Scer\GAL4^ey.PH