FlyBase Allele Report: Dmel\mei-217[r1]

General Information

Symbol

Dmel\mei-217^r1

Species

D. melanogaster

Name

FlyBase ID

FBal0105005

Feature type

allele

Associated gene

Dmel\mei-217

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Liu et al., 2000)

Progenitor genotype

Cytology

Description

The A to T mutation in mei-217^r1 was originally thought to be in the 5' UTR, "42bp upstream of the AUG codon" (FBrf0127203). However, it appears that the mei-217 open reading frame begins with an ATG 250bp upstream of the start codon previously predicted in FBrf0127203, which would mean that the mutation is actually within the coding sequence (and changes a Lys residue to a stop codon).

(Manheim et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:17,142,655..17,142,655 [-]

Nucleotide change:

A17142655T

Reported nucleotide change:

A?T

Amino acid change:

K39term | mei-217-PC

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement: Mutation is 42bp upstream of the AUG codon of the open reading frame of mei-217.

(Liu et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | recessive

(Liu et al., 2000)

Phenotype Manifest In

meiosis I | female

(Liu et al., 2000)

Detailed Description

Statement

Reference

Mutants show an elevated frequency of X chromosome nondisjunction in females. Most or all of the nondisjunction events involve achiasmate chromosomes in the first meiotic division. An equal number of diplo-X and nullo-X progeny are seen, indicating that meiotic chromosome loss is not a significant factor. Second chromosome nondisjunction is also elevated. The gametic frequency of simultaneous X and second chromosome nondisjunction is greater than 62%. The frequency of crossing over is reduced in homozygous females. Crossing over is reduced by a similar degree on both the X chromosome and the left arm of the second chromosome. The reduction in crossing over is not uniform along each chromosome arm. On the second chromosome, crossing over is reduced more in distal regions rather than in proximal regions. Precocious anaphase is seen in homozygous mature oocytes.

(Liu et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mei-217^r1 has abnormal meiotic cell cycle | recessive phenotype, suppressible by mei-41^D18

(Liu et al., 2000)

Enhancer of

Statement

Reference

mei-217^r1 is an enhancer of female sterile | recessive phenotype of spn-B^BU

(Liu et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

mei-217^r1 has meiosis I | female phenotype, suppressible by mei-41^D18

(Liu et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

In mei-217^r1 mei-41^D18 double mutants a metaphase arrest is almost always seen in mature oocytes (as occurs in wild type).

(Liu et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation with an elevated frequency of X chromosome nondisjunction in females.

(Liu et al., 2000)

Comments

"Amino acid replacement: K?@." was stated as revision.

(Manheim et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mei-217^r1

(Liu et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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