FlyBase Allele Report: Dmel\PolA1[Emus304]

General Information

Symbol

Dmel\PolA1^Emus304

Species

D. melanogaster

Name

FlyBase ID

FBal0105216

Feature type

allele

Associated gene

Dmel\PolA1

Associated Insertion(s)

Carried in Construct

Also Known As

E(mus304)

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Brodsky et al., 2000)

Progenitor genotype

Cytology

Description

Single base pair deletion in the third exon at codon 301, which results in a frameshift and a premature stop codon 29 codons downstream.

(LaRocque et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:21,670,633..21,670,633 [-]

Comment:

Loss of an "A" base in codon Q301 causes a frameshift and early translation termination 29 codons downstream.

(LaRocque et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with PolA1^Q1132ter)

(LaRocque et al., 2007)

lethal (with PolA1^Q1322ter)

(LaRocque et al., 2007)

lethal | recessive

(Brodsky et al., 2000)

lethal - all die during embryonic stage | recessive

(LaRocque et al., 2007)

some die during embryonic stage | recessive

(LaRocque et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Heterozygotes show no increase in loss of heterozygosity compared to wild type.

(LaRocque et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E(mus304)[+]/PolA1^Emus304, mei-41^29D has visible phenotype, suppressible by p53^5A-1-4/p53^5A-1-4

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has increased cell death phenotype, suppressible by p53^5A-1-4/p53^5A-1-4

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has visible phenotype, suppressible by CycB[+]/CycB²

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has visible phenotype, suppressible by CycA^C8LR1/CycA[+]

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has increased cell death phenotype, suppressible by CycA^C8LR1/CycA[+]

(LaRocque et al., 2007)

Enhancer of

Statement

Reference

E(mus304)[+]/PolA1^Emus304 is an enhancer of visible phenotype of Df(3L)W4/mus304^D2

(Brodsky et al., 2000)

Other

Statement

Reference

E(mus304)[+]/PolA1^Emus304, lok³⁰ has increased cell death phenotype

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, grp²⁰⁹/grp^Z5170 has visible | male limited phenotype

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has visible phenotype

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has visible | recessive phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has visible phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has visible phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mei-41^29D has visible phenotype

(Brodsky et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

E(mus304)[+]/PolA1^Emus304, mei-41^29D has eye phenotype, suppressible by p53^5A-1-4/p53^5A-1-4

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has eye phenotype, suppressible by CycB[+]/CycB²

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has eye phenotype, suppressible by CycA^C8LR1/CycA[+]

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has wing disc phenotype, suppressible by CycA^C8LR1/CycA[+]

(LaRocque et al., 2007)

Enhancer of

Statement

Reference

E(mus304)[+]/PolA1^Emus304 is an enhancer of macrochaeta phenotype of Df(3L)W4/mus304^D2

(Brodsky et al., 2000)

Other

Statement

Reference

E(mus304)[+]/PolA1^Emus304, mei-41^29D has eye phenotype

(LaRocque et al., 2007, Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has ommatidium phenotype

(LaRocque et al., 2007, Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, lok³⁰ has wing disc phenotype

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, grp²⁰⁹/grp^Z5170 has eye | male limited phenotype

(LaRocque et al., 2007)

E(mus304)[+]/PolA1^Emus304, mei-41^29D has macrochaeta phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has eye phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mei-41^29D has macrochaeta phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mei-41^29D has eye phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mei-41^29D has ommatidium phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has eye phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype

(Brodsky et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

mei-41^29D mutants that are also heterozygous for DNApol-α180^Emus304 have a rough eye phenotype that includes fused ommatidia and tissue loss.

Heterozygosity for DNApol-α180^Emus304 further increases the average number of apoptotic cells per larval wing disc is increased fourfold in mei-41^29D/Y flies.

p53^5A-1-4/p53^5A-1-4 completely suppresses the rough eye phenotype of DNApol-α180^Emus304/+ ; mei-41^29D/Y flies. The increased level of apoptosis seen in DNApol-α180^Emus304/+ ; mei-41^29D/Y wing discs is restored by p53^5A-1-4/p53^5A-1-4 to the level seen in mei-41^29D/Y single mutants.

Heterozygosity for DNApol-α180^Emus304 further increases the increase in loss of heterozygosity seen in mei-41^29D/Y flies. There is a significant increase in the frequency of mitotic crossovers in the e to st region of the third chromosome in the double mutants.

The rough eye phenotype seen in mei-41^29D ; DNApol-α180^Emus304/+ adults is partially rescued by CycB²/+ and completely rescued by CycA^C8LR1/+. The increased apoptosis seen in the wing discs of mei-41^29D ; DNApol-α180^Emus304/+ mutants is rescued by CycA^C8LR1/+ to levels indistinguishable from those of mei-41^29D single mutants or mei-41^29D ; CycA^C8LR1/+ mutants.

The eyes of lok³⁰ ; DNApol-α180^Emus304/+ flies are indistinguishable from wild type. The wing discs of these animals show a slight increase in apoptosis compared to wild type.

grp²⁰⁹/grp^Z5170 ; DNApol-α180^Emus304/+ males have a rough eye phenotype, while grp²⁰⁹/grp^Z5170 ; DNApol-α180^Emus304/+ females have wild-type eyes. The wing discs of these mutant animals show an increase in apoptosis compared to wild type.

(LaRocque et al., 2007)

mus304^D1/mus304^D2 flies have wild-type eyes. If the flies are also heterozygous for E(mus304)¹ they have rough eyes and abnormal or missing bristles. Homozygous mei-41^29D flies have wild-type eyes. If the flies are also heterozygous for E(mus304)¹ they have rough eyes and missing bristles. mus304^D2/Df(3L)W4 animals have macrochaetae defects (probably due genomic instability resulting in the loss of genetic material at haploinsufficient Minute loci). The frequency of defects are increased by one copy of E(mus304)¹.

(Brodsky et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

PolA1^Q1322ter

(LaRocque et al., 2007)

PolA1^Q1132ter

(LaRocque et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: mus304^D2.

(Brodsky et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DNA Polα-180^Emus304

(Blythe and Wieschaus, 2015)

DNApol-α180^Emus304

(Lafave et al., 2014)

E(mus304)

(LaRocque et al., 2007, Brodsky et al., 2000)

E(mus304)¹

PolA1^Emus304

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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