FlyBase Allele Report: Dmel\pAbp[UAS.cSa]

General Information

Symbol

Dmel\pAbp^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sigrist

FlyBase ID

FBal0117408

Feature type

allele

Associated gene

Dmel\pAbp

Associated Insertion(s)

Carried in Construct

P{UAS-pAbp.S}

Also Known As

UAS-PABP

Key Links

Transgenic product class

wild_type

(Sigrist et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sigrist et al., 2000)

Mutagen

in vitro construct

(Sigrist et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-pAbp.S}

Cytology

Description

UASt regulatory sequences drive expression of the pAbp coding region (without the 5' and 3' UTRs).

(Sigrist et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

pAbp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cEa

(Kim et al., 2014)

modeled by Hsap\ATXN2^32Q.UAS

(Kim et al., 2014)

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Lessing and Bonini, 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

abnormal neurophysiology, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

Phenotype Manifest In

bouton, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

larval multidendritic class IV neuron | somatic clone, with Scer\GAL4^Act.PU

(Cziko et al., 2009)

neuromuscular junction, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2000)

Detailed Description

Statement

Reference

Over-expression of pAbp^Scer\UAS.cSa reduces dendrite branching, and alters dendritic morphology in larval class IV sensory neurons (induced under the control of Scer\GAL4^Act.PU). Total dendrite length is significantly reduced in these neurons.

(Cziko et al., 2009)

The mean amplitude of miniature postsynaptic currents (mEJCs) at the neuromuscular junction is indistinguishable from wild type in flies expressing pAbp^Scer\UAS.cSa under the control of Scer\GAL4^Mhc.PW. Evoked postsynaptic currents (eEJCs) are significantly larger than in wild-type. The number of junctional boutons per neuromuscular junction is increased compared to wild type.

(Sigrist et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pAbp^UAS.cSa, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Hsap\TARDBP^UAS.cEa, Scer\GAL4^GMR.PU

(Kim et al., 2014)

Suppressor of

Statement

Reference

pAbp^UAS.cSa, Scer\GAL4^ninaE.PD is a suppressor | partially of decreased cell number | conditional phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^ninaE.PD

(Lessing and Bonini, 2008)

pAbp^UAS.cSa, Scer\GAL4^ninaE.PD is a suppressor | partially of decreased cell number | conditional phenotype of Atx2^UAS.cSa, Scer\GAL4^ninaE.PD

(Lessing and Bonini, 2008)

pAbp^UAS.cSa, Scer\GAL4^Bx-MS1096 is a suppressor of abnormal cell growth phenotype of Paip2^UAS.Tag:HA, Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

pAbp^UAS.cSa, Scer\GAL4^Bx-MS1096 is a suppressor of visible phenotype of Paip2^UAS.Tag:HA, Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

Other

Statement

Reference

Hsap\ATXN2^32Q.UAS, Scer\GAL4^GMR.PU, pAbp^UAS.cSa has visible phenotype

(Kim et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

pAbp^UAS.cSa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\TARDBP^UAS.cEa, Scer\GAL4^GMR.PU

(Kim et al., 2014)

Suppressor of

Statement

Reference

pAbp^UAS.cSa, Scer\GAL4^ninaE.PD is a suppressor | partially of photoreceptor | conditional phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^ninaE.PD

(Lessing and Bonini, 2008)

pAbp^UAS.cSa, Scer\GAL4^ninaE.PD is a suppressor | partially of photoreceptor | conditional phenotype of Atx2^UAS.cSa, Scer\GAL4^ninaE.PD

(Lessing and Bonini, 2008)

pAbp^UAS.cSa, Scer\GAL4^Bx-MS1096 is a suppressor of wing disc phenotype of Paip2^UAS.Tag:HA, Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

pAbp^UAS.cSa, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Paip2^UAS.Tag:HA, Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

Other

Statement

Reference

Hsap\ATXN2^32Q.UAS, Scer\GAL4^GMR.PU, pAbp^UAS.cSa has eye phenotype

(Kim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of pAbp^Scer\UAS.cSa partially suppresses the visible photoreceptor loss seen when Atx2^Scer\UAS.cSa is expressed in differentiated photoreceptor neurons under the control of Scer\GAL4^ninaE.PD. On average 5.1 photoreceptors are seen per ommatidium in 18 day old flies, as opposed to the seven seen in wild type. This compares to 2.9 when Atx2^Scer\UAS.cSa is expressed alone.

(Lessing and Bonini, 2008)

Xenogenetic Interactions

Statement

Reference

Expression of pAbp^Scer\UAS.cSa enhances the eye phenotype seen when Hsap\TARDBP^Scer\UAS.cEa is expressed under the control of Scer\GAL4^GMR.PU.

Expression of pAbp^Scer\UAS.cSa produces an eye phenotype when co-expressed with Hsap\ATXN2^32Q.Scer\UAS under the control of Scer\GAL4^GMR.PU.

Co-expression of pAbp^Scer\UAS.cSa and Hsap\ATXN2^{32Q.ΔPAM2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU does not induce retinal degeneration.

(Kim et al., 2014)

Expression of pAbp^Scer\UAS.cSa partially suppresses the photoreceptor loss seen when Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} is expressed in differentiated photoreceptor neurons under the control of Scer\GAL4^ninaE.PD. On average 6.0 visible photoreceptors are seen per ommatidium in 18 day old flies, as opposed to the seven seen in wild type. This compares to 4.2 when Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} is expressed alone.

(Lessing and Bonini, 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9148

w^*; P{UAS-pAbp.S}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pAbp^Scer\UAS.cSa

pAbp^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sigrist

(Sigrist et al., 2000)

Secondary FlyBase IDs

References (7)

Report Sections

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