FlyBase Allele Report: Hsap\GLI3[UAS.Tag:MYC]

General Information

Symbol

Hsap\GLI3^UAS.Tag:MYC

Species

H. sapiens

Name

FlyBase ID

FBal0119473

Feature type

allele

Associated gene

Hsap\GLI3

Associated Insertion(s)

Carried in Construct

P{UAS-Myc-Hsap\GLI3}

Key Links

Transgenic product class

cDNA

(Aza-Blanc et al., 2000)

wild_type

(Aza-Blanc et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Aza-Blanc et al., 2000)

wild_type

(Aza-Blanc et al., 2000)

Mutagen

in vitro construct

(Aza-Blanc et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Myc-Hsap\GLI3}

Cytology

Description

UASt regulatory sequences drive expression of a Hsap\GLI3 cDNA tagged with six N-terminal Tag:MYC tags.

(Aza-Blanc et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\GLI3

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Phenotype Manifest In

wing vein L3, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

wing vein L4, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Detailed Description

Statement

Reference

Expression of Hsap\GLI3^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 does not alter wing vein morphology or growth. Expression of Hsap\GLI3^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^en-e16E does not induce growth or vein pattern alteration in the wing, although wing veins 3 and 4 are slightly fused.

(Aza-Blanc et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E has visible phenotype, enhanceable by ptc^UAS.cJa, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Enhancer of

Statement

Reference

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E is an enhancer of visible phenotype of Scer\GAL4^en-e16E, ptc^UAS.cJa

(Aza-Blanc et al., 2000)

Other

Statement

Reference

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E, ptc^UAS.cJa has lethal phenotype

(Aza-Blanc et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E has wing vein L3 phenotype, enhanceable by ptc^UAS.cJa, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E has wing vein L4 phenotype, enhanceable by ptc^UAS.cJa, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E has wing phenotype, enhanceable by ptc^UAS.cJa, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Enhancer of

Statement

Reference

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E is an enhancer of wing vein L3 phenotype of Scer\GAL4^en-e16E, ptc^UAS.cJa

(Aza-Blanc et al., 2000)

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E is an enhancer of wing vein L4 phenotype of Scer\GAL4^en-e16E, ptc^UAS.cJa

(Aza-Blanc et al., 2000)

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E is an enhancer of wing phenotype of Scer\GAL4^en-e16E, ptc^UAS.cJa

(Aza-Blanc et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of both Hsap\GLI3^{Scer\UAS.T:Hsap\MYC} and ptc^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E at 17^oC results in an enhancing, synergistic effect; the wings are significantly reduced in size. The central region of the wing, including veins 3 and 4 is absent. Higher levels of expression of both Hsap\GLI3^{Scer\UAS.T:Hsap\MYC} and ptc^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E result in lethality.

(Aza-Blanc et al., 2000)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells to study the behaviour of the Hsap\GLI3 protein in these cells.

(Aza-Blanc et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\GLI3^{Scer\UAS.T:Hsap\MYC}

Hsap\GLI3^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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