The increased division of intestinal stem cells in the adult posterior midgut during normal homeostasis characteristic for Sulf1KO;Cherry/Sulf1ΔP1 mutants can be suppressed by expression of ptcScer\UAS.cJa under the control of Scer\GAL4NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood).
Co-expression of ptcScer\UAS.cJa significantly suppresses the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with TkR99DScer\UAS.T:Avic\GFP-EGFP over-expressed by Scer\GAL4ppk.1.9.
When both ptcScer\UAS.cJa and dallysec.Scer\UAS.T:Hsap\MYC are expressed under the control of Scer\GAL4A9, the dallysec.Scer\UAS.T:Hsap\MYC large body phenotype is suppressed, but the ptcScer\UAS.cJa wing phenotype remains.
ptcScer\UAS.cJa Scer\GAL471B flies carrying a relatively weakly expressing hhScer\UAS.cCa insertion line have wing discs that are indistinguishable from wild-type. ptcScer\UAS.cJa Scer\GAL471B flies carrying a more strongly expressing hhScer\UAS.cCa insertion line lack all veins in the anterior compartment, while vein 4 is restored. Ectopic expression of dppScer\UAS.cCa in ptcScer\UAS.cJa Scer\GAL471B flies does not restore either presumptive veins 3 and 4 or the Sc25, L3 or ACV sensilla precursors. Most of the wing tissue, except vein 3 and its associated sensilla and vein 4, is recovered in ptcScer\UAS.cJa Scer\GAL4MD-638 flies also carrying dppScer\UAS.cCa. Flies expressing both ptcScer\UAS.cJa and hhScer\UAS.cCa using Scer\GAL4MD-638 show recovery of the posterior compartment veins, ectopic induction of vein 3 and L3 sensilla and an enlargement of the region between veins 3 and 4.
In a hhMrt background wing vein deletions are suppressed to mild vein fusions, hhMrtr1 and hhMrtr2 fail to suppress the wing vein deletion phenotype. In a Df(4)M101-62f or ciCe-1 the wing phenotype is enhanced.
The wing vein deletions caused by expression of ptcScer\UAS.cJa under the control of Scer\GAL471B are suppressed to mild vein fusions in a hhMrt background, whereas hhMrtr1 and hhMrtr2 fail to suppress the phenotype. Df(4)M101-62f or ciCe-1 enhance the ptcScer\UAS.cJa; Scer\GAL471B wing phenotype.
Expression of both Hsap\GLI3Scer\UAS.T:Hsap\MYC and ptcScer\UAS.cJa under the control of Scer\GAL4en-e16E at 17oC results in an enhancing, synergistic effect; the wings are significantly reduced in size. The central region of the wing, including veins 3 and 4 is absent. Higher levels of expression of both Hsap\GLI3Scer\UAS.T:Hsap\MYC and ptcScer\UAS.cJa under the control of Scer\GAL4en-e16E result in lethality.