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General Information
Symbol
Dmel\ptcUAS.cJa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Johnson
FlyBase ID
FBal0050312
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ptc, UAS:Ptc, UAS ptc B1, UAS ptc
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a 4kb EcoRI-SpeI fragment of ptc cDNA, containing the entire coding region, is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

tergite & macrochaeta, with Scer\GAL4hs.PB

Detailed Description
Statement
Reference

Expression of ptcScer\UAS.cJa under the control of Scer\GAL4Tab2-201Y results in a weak pruning defect in the mushroom body gamma neurons in approximately 13% of brains.

Expression of ptcScer\UAS.cJa under the control of Scer\GAL4GMR71G10 results in a moderate pruning defect in the mushroom body gamma neurons in approximately 72% of brains.

Expression of ptcScer\UAS.cJa under the control of Scer\GAL4GMR16A06 results in a moderate pruning defect in the mushroom body gamma neurons in approximately 68% of brains.

Expression of ptcScer\UAS.cJa under the control of Scer\GAL4en-e16E reduces the 3-4 intervein region in the wing.

Expression of ptcScer\UAS.cJa under the control of Scer\GAL4pb.PJ does not affect labial palp development when flies are raised at 22oC. However, when flies of the same genotype are raised at 28oC, some pseudotracheal rows near the A-P boundary of the labium are absent.

Expression of ptcScer\UAS.cJa, under the control of Scer\GAL4A9, causes a reduction in the amount of space between longitudinal veins L3 and L4 and the absence of the anterior crossvein in the wing.

When ptcScer\UAS.cJa is driven by Scer\GAL471B reduces wing size.

ptcScer\UAS.cJa when driven by Scer\GAL4C-765 leads to a reduced wing phenotype.

When ptcScer\UAS.cJa is driven by Scer\GAL4en-e16E wings are about 8% smaller than wild-type but not severely misshapen.

Expression of ptcScer\UAS.cJa under the control of Scer\GAL4en-e16E at 17oC results in a slight fusion between wing veins L3 and L4.

When ptcScer\UAS.cJa is driven by Scer\GAL471B results in defects in the anterior posterior boundary in the adult wing. Phenotypes range from a weak effect, partial fusion of wing veins 3 and 4 to as stronger effect, the deletion of the region between veins 2 and 4. When ptcScer\UAS.cJa is driven by Scer\GAL4en-e16E, wing veins 3 and 4 become partially fused.

Overexpression of ptcScer\UAS.cJa driven by either Scer\GAL4dpp.blk1 or Scer\GAL4en-e16E, causes the partial loss of L3-L4 intervein.

When ptcScer\UAS.cJa is driven by Scer\GAL471B at 17oC the adult wing has a missing anterior crossvein. At higher temperature parts of L3 and L4 are deleted.

Veins L3 and L4 are closer together in the wings of flies expressing ptcScer\UAS.cJa under the control of Scer\GAL4en-e16E than in wild-type flies. The proximal part of vein L4 is often missing.

Ubiquitous expression of ptcScer\UAS.cJa in the pupal abdomen using Scer\GAL4hs.PB results in a number of phenotypes, including loss of the tergite pigment band, transformation of tergite macrochaetae to microchaetae and complete of partial loss of the tergite posterior hairy zone (PHZ) and intersegmental membrane (ISM).

Causes deletion of presumptive wing veins 3 and 4 and the region between them in the wing disc, when expressed using Scer\GAL471B at 29oC. Flies expressing ptcScer\UAS.cJa using Scer\GAL4MD-638 lack most of the wing, except proximomarginal structures.

In the presence of Scer\GAL471B the wing phenotype varies from fusion of L3 and L4 veins at the proximal end and the absence of the anterior cross vein (ACV) to L3 and L4 veins fused at the distal end or become progressively deleted and altered posterior cross vein. In the extreme L3 and L4 are almost entirely missing and L2 and L5 are partially deleted. Wing morphology is maintained but the overall size progressively decreases.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The increased division of intestinal stem cells in the adult posterior midgut during normal homeostasis characteristic for Sulf1KO;Cherry/Sulf1ΔP1 mutants can be suppressed by expression of ptcScer\UAS.cJa under the control of Scer\GAL4NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood).

Co-expression of ptcScer\UAS.cJa significantly suppresses the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with TkR99DScer\UAS.T:Avic\GFP-EGFP over-expressed by Scer\GAL4ppk.1.9.

Expression of ptcScer\UAS.cJa under the control of either Scer\GAL4Tab2-201Y or Scer\GAL4GMR71G10 enhances the pruning defect seen in UvragLL03097 mushroom body gamma neuron clones.

Co-expression of ptcScer\UAS.cJa does not suppress the wing defects caused by expression of fuScer\UAS.T:Hsap\Myr2,T:Avic\GFP-CFP under the control of Scer\GAL4Bx-MS1096.

When both ptcScer\UAS.cJa and dallysec.Scer\UAS.T:Hsap\MYC are expressed under the control of Scer\GAL4A9, the dallysec.Scer\UAS.T:Hsap\MYC large body phenotype is suppressed, but the ptcScer\UAS.cJa wing phenotype remains.

The addition of vnL6 enhances the wing phenotype caused by ptcScer\UAS.cJa driven by Scer\GAL471B. The addition of vnScer\UAS.cSa suppresses the phenotype, restoring the anterior crossvein.

ptcScer\UAS.cJa Scer\GAL471B flies carrying a relatively weakly expressing hhScer\UAS.cCa insertion line have wing discs that are indistinguishable from wild-type. ptcScer\UAS.cJa Scer\GAL471B flies carrying a more strongly expressing hhScer\UAS.cCa insertion line lack all veins in the anterior compartment, while vein 4 is restored. Ectopic expression of dppScer\UAS.cCa in ptcScer\UAS.cJa Scer\GAL471B flies does not restore either presumptive veins 3 and 4 or the Sc25, L3 or ACV sensilla precursors. Most of the wing tissue, except vein 3 and its associated sensilla and vein 4, is recovered in ptcScer\UAS.cJa Scer\GAL4MD-638 flies also carrying dppScer\UAS.cCa. Flies expressing both ptcScer\UAS.cJa and hhScer\UAS.cCa using Scer\GAL4MD-638 show recovery of the posterior compartment veins, ectopic induction of vein 3 and L3 sensilla and an enlargement of the region between veins 3 and 4.

In a hhMrt background wing vein deletions are suppressed to mild vein fusions, hhMrtr1 and hhMrtr2 fail to suppress the wing vein deletion phenotype. In a Df(4)M101-62f or ciCe-1 the wing phenotype is enhanced.

The wing vein deletions caused by expression of ptcScer\UAS.cJa under the control of Scer\GAL471B are suppressed to mild vein fusions in a hhMrt background, whereas hhMrtr1 and hhMrtr2 fail to suppress the phenotype. Df(4)M101-62f or ciCe-1 enhance the ptcScer\UAS.cJa; Scer\GAL471B wing phenotype.

Xenogenetic Interactions
Statement
Reference

Expression of both Hsap\GLI3Scer\UAS.T:Hsap\MYC and ptcScer\UAS.cJa under the control of Scer\GAL4en-e16E at 17oC results in an enhancing, synergistic effect; the wings are significantly reduced in size. The central region of the wing, including veins 3 and 4 is absent. Higher levels of expression of both Hsap\GLI3Scer\UAS.T:Hsap\MYC and ptcScer\UAS.cJa under the control of Scer\GAL4en-e16E result in lethality.

Complementation and Rescue Data
Partially rescues
Comments

The ptcD584N.Scer\UAS wing phenotype is suppressed by coexpression ptcScer\UAS.cJa (when driven by Scer\GAL471B).

The addition of ptcScer\UAS.cJa (when driven by Scer\GAL4da.G32) to ptc16 embryos complements the cuticle defects of these embryos - instead of 20% of embryos having a "ptc" phenotype, only 1% do.

Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ptcScer\UAS.cJa
ptcUAS.cJa
Name Synonyms
Saccharomyces cerevisiae UAS construct of Johnson
Secondary FlyBase IDs
    References (34)