FlyBase Allele Report: Dmel\ptc[UAS.cJa]

General Information

Symbol

Dmel\ptc^UAS.cJa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Johnson

FlyBase ID

FBal0050312

Feature type

allele

Associated gene

Dmel\ptc

Associated Insertion(s)

Carried in Construct

P{UAS-ptc.J}

Also Known As

UAS-ptc, UAS:Ptc, UAS ptc B1, UAS ptc

Key Links

Transgenic product class

cDNA

(Johnson et al., 1995)

wild_type

(Johnson et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Johnson et al., 1995)

wild_type

(Johnson et al., 1995)

Mutagen

in vitro construct

(Biehs et al., 1998, Kopp et al., 1997, Johnson et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-ptc.J}

Cytology

Description

Expression of a 4kb EcoRI-SpeI fragment of ptc cDNA, containing the entire coding region, is expressed under the control of UASt regulatory sequences.

(Johnson et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ptc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GMR16A06

(Issman-Zecharya and Schuldiner, 2014)

abnormal neuroanatomy, with Scer\GAL4^GMR71G10

(Issman-Zecharya and Schuldiner, 2014)

abnormal neuroanatomy, with Scer\GAL4^Tab2-201Y

(Issman-Zecharya and Schuldiner, 2014)

decreased cell number | larval stage, with Scer\GAL4^btl.PS

(Hatori and Kornberg, 2020)

decreased occurrence of cell division | larval stage, with Scer\GAL4^btl.PS

(Hatori and Kornberg, 2020)

decreased size | adult stage, with Scer\GAL4^btl.PS

(Hatori and Kornberg, 2020)

decreased size | larval stage, with Scer\GAL4^btl.PS

(Hatori and Kornberg, 2020)

visible, with Scer\GAL4^71B

(Wessells et al., 1999)

visible, with Scer\GAL4^Bx-MS1096

(Claret et al., 2007)

visible, with Scer\GAL4^C-765

(Martin et al., 2001)

visible, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000, Biehs et al., 1998)

visible, with Scer\GAL4^hs.PB

(Kopp et al., 1997)

Phenotype Manifest In

abdominal tergite, with Scer\GAL4^hs.PB

(Kopp et al., 1997)

air sac primordium | larval stage, with Scer\GAL4^btl.PS

(Hatori and Kornberg, 2020)

anterior crossvein, with Scer\GAL4^71B

(Wessells et al., 1999, Johnson et al., 1995)

anterior crossvein, with Scer\GAL4^A9

(Takeo et al., 2005)

cytoneme | decreased number | larval stage, with Scer\GAL4^btl.PS

(Hatori and Kornberg, 2020)

dorsal air sac | adult stage, with Scer\GAL4^btl.PS

(Hatori and Kornberg, 2020)

first posterior wing cell, with Scer\GAL4^71B

(Johnson et al., 2000)

first posterior wing cell, with Scer\GAL4^dpp.blk1

(Vervoort et al., 1999)

first posterior wing cell, with Scer\GAL4^en-e16E

(Lu et al., 2006, Vervoort et al., 1999)

gamma Kenyon cell, with Scer\GAL4^GMR16A06

(Issman-Zecharya and Schuldiner, 2014)

gamma Kenyon cell, with Scer\GAL4^GMR71G10

(Issman-Zecharya and Schuldiner, 2014)

gamma Kenyon cell, with Scer\GAL4^Tab2-201Y

(Issman-Zecharya and Schuldiner, 2014)

posterior crossvein, with Scer\GAL4^71B

(Johnson et al., 1995)

pseudotrachea | heat sensitive, with Scer\GAL4^pb.PJ

(Joulia et al., 2005)

submarginal wing cell, with Scer\GAL4^71B

(Johnson et al., 2000)

tergite & macrochaeta, with Scer\GAL4^hs.PB

(Kopp et al., 1997)

wing, with Scer\GAL4^71B

(Zhu et al., 2003, Wessells et al., 1999, Johnson et al., 1995)

wing, with Scer\GAL4^A9

(Takeo et al., 2005)

wing, with Scer\GAL4^Bx-MS1096

(Claret et al., 2007)

wing, with Scer\GAL4^C-765

(Martin et al., 2001)

wing, with Scer\GAL4^en-e16E

(Ramirez-Weber et al., 2001, Biehs et al., 1998)

wing, with Scer\GAL4^MD-638

(Mullor et al., 1997)

wing disc | larval stage, with Scer\GAL4¹¹⁵¹

(Hatori and Kornberg, 2020)

wing vein, with Scer\GAL4^71B

(Wessells et al., 1999)

wing vein L2, with Scer\GAL4^71B

(Johnson et al., 1995)

wing vein L3, with Scer\GAL4^71B

(Johnson et al., 2000, Wessells et al., 1999, Johnson et al., 1995)

wing vein L3, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000, Johnson et al., 2000, Biehs et al., 1998)

wing vein L3 | precursor, with Scer\GAL4^71B

(Mullor et al., 1997)

wing vein L3 | precursor, with Scer\GAL4^MD-638

(Mullor et al., 1997)

wing vein L4, with Scer\GAL4^71B

(Johnson et al., 2000, Wessells et al., 1999, Johnson et al., 1995)

wing vein L4, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000, Johnson et al., 2000)

wing vein L4 | precursor, with Scer\GAL4^71B

(Mullor et al., 1997)

wing vein L5, with Scer\GAL4^71B

(Johnson et al., 1995)

Detailed Description

Statement

Reference

Expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^Tab2-201Y results in a weak pruning defect in the mushroom body gamma neurons in approximately 13% of brains.

Expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^GMR71G10 results in a moderate pruning defect in the mushroom body gamma neurons in approximately 72% of brains.

Expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^GMR16A06 results in a moderate pruning defect in the mushroom body gamma neurons in approximately 68% of brains.

(Issman-Zecharya and Schuldiner, 2014)

Expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E reduces the 3-4 intervein region in the wing.

(Lu et al., 2006)

Expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^pb.PJ does not affect labial palp development when flies are raised at 22^oC. However, when flies of the same genotype are raised at 28^oC, some pseudotracheal rows near the A-P boundary of the labium are absent.

(Joulia et al., 2005)

Expression of ptc^Scer\UAS.cJa, under the control of Scer\GAL4^A9, causes a reduction in the amount of space between longitudinal veins L3 and L4 and the absence of the anterior crossvein in the wing.

(Takeo et al., 2005)

When ptc^Scer\UAS.cJa is driven by Scer\GAL4^71B reduces wing size.

(Zhu et al., 2003)

ptc^Scer\UAS.cJa when driven by Scer\GAL4^C-765 leads to a reduced wing phenotype.

(Martin et al., 2001)

When ptc^Scer\UAS.cJa is driven by Scer\GAL4^en-e16E wings are about 8% smaller than wild-type but not severely misshapen.

(Ramirez-Weber et al., 2001)

Expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E at 17^oC results in a slight fusion between wing veins L3 and L4.

(Aza-Blanc et al., 2000)

When ptc^Scer\UAS.cJa is driven by Scer\GAL4^71B results in defects in the anterior posterior boundary in the adult wing. Phenotypes range from a weak effect, partial fusion of wing veins 3 and 4 to as stronger effect, the deletion of the region between veins 2 and 4. When ptc^Scer\UAS.cJa is driven by Scer\GAL4^en-e16E, wing veins 3 and 4 become partially fused.

(Johnson et al., 2000)

Overexpression of ptc^Scer\UAS.cJa driven by either Scer\GAL4^dpp.blk1 or Scer\GAL4^en-e16E, causes the partial loss of L3-L4 intervein.

(Vervoort et al., 1999)

When ptc^Scer\UAS.cJa is driven by Scer\GAL4^71B at 17^oC the adult wing has a missing anterior crossvein. At higher temperature parts of L3 and L4 are deleted.

(Wessells et al., 1999)

Veins L3 and L4 are closer together in the wings of flies expressing ptc^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E than in wild-type flies. The proximal part of vein L4 is often missing.

(Biehs et al., 1998)

Ubiquitous expression of ptc^Scer\UAS.cJa in the pupal abdomen using Scer\GAL4^hs.PB results in a number of phenotypes, including loss of the tergite pigment band, transformation of tergite macrochaetae to microchaetae and complete of partial loss of the tergite posterior hairy zone (PHZ) and intersegmental membrane (ISM).

(Kopp et al., 1997)

Causes deletion of presumptive wing veins 3 and 4 and the region between them in the wing disc, when expressed using Scer\GAL4^71B at 29^oC. Flies expressing ptc^Scer\UAS.cJa using Scer\GAL4^MD-638 lack most of the wing, except proximomarginal structures.

(Mullor et al., 1997)

In the presence of Scer\GAL4^71B the wing phenotype varies from fusion of L3 and L4 veins at the proximal end and the absence of the anterior cross vein (ACV) to L3 and L4 veins fused at the distal end or become progressively deleted and altered posterior cross vein. In the extreme L3 and L4 are almost entirely missing and L2 and L5 are partially deleted. Wing morphology is maintained but the overall size progressively decreases.

(Johnson et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, ptc^UAS.cJa has visible phenotype, enhanceable by Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^Tab2-201Y/ptc^UAS.cJa is an enhancer of abnormal neuroanatomy | somatic clone phenotype of Uvrag^LL03097

(Issman-Zecharya and Schuldiner, 2014)

ptc^UAS.cJa/Scer\GAL4^GMR71G10 is an enhancer of abnormal neuroanatomy | somatic clone phenotype of Uvrag^LL03097

(Issman-Zecharya and Schuldiner, 2014)

ptc^UAS.cJa, Scer\GAL4^en-e16E is an enhancer of visible phenotype of Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^NP6267/ptc^UAS.cJa is a suppressor of increased occurrence of cell division | adult stage phenotype of Sulf1^KO.Cherry/Sulf1^ΔP1

(Takemura and Nakato, 2017)

ptc^UAS.cJa/Scer\GAL4^ppk.1.9 is a suppressor | partially of abnormal pain response | early third instar larval stage | heat sensitive phenotype of Scer\GAL4^ppk.1.9, TkR99D^UAS.EGFP

(Im et al., 2015)

Scer\GAL4^A9/ptc^UAS.cJa is a suppressor of increased body size phenotype of Scer\GAL4^A9, dally^{sec.UAS.Tag:MYC}

(Takeo et al., 2005)

NOT Suppressor of

Statement

Reference

ptc^UAS.cJa/Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, fu^{UAS.Tag:Palm(hGAP43),CFP}

(Claret et al., 2007)

Other

Statement

Reference

Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E, ptc^UAS.cJa has lethal phenotype

(Aza-Blanc et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, ptc^UAS.cJa has wing vein L3 phenotype, enhanceable by Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Scer\GAL4^en-e16E, ptc^UAS.cJa has wing vein L4 phenotype, enhanceable by Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Scer\GAL4^en-e16E, ptc^UAS.cJa has wing phenotype, enhanceable by Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Scer\GAL4^71B, ptc^UAS.cJa has wing phenotype, enhanceable by vn^L6

(Wessells et al., 1999)

Scer\GAL4^71B, ptc^UAS.cJa has wing vein phenotype, enhanceable by vn^L6

(Wessells et al., 1999)

Scer\GAL4^71B, ptc^UAS.cJa has wing phenotype, enhanceable by Df(4)M101-62f

(Johnson et al., 1995)

Scer\GAL4^71B, ptc^UAS.cJa has wing phenotype, enhanceable by ci^Ce-1

(Johnson et al., 1995)

Suppressed by

Statement

Reference

Scer\GAL4^71B, ptc^UAS.cJa has anterior crossvein phenotype, suppressible by Scer\GAL4^71B/vn^UAS.cSa

(Wessells et al., 1999)

Scer\GAL4^MD-638, ptc^UAS.cJa has wing phenotype, suppressible | partially by Scer\GAL4^MD-638/dpp^UAS.cCa

(Mullor et al., 1997)

Scer\GAL4^71B, ptc^UAS.cJa has wing vein phenotype, suppressible by hh^Mrt

(Johnson et al., 1995)

NOT suppressed by

Statement

Reference

Scer\GAL4^A9, ptc^UAS.cJa has anterior crossvein phenotype, non-suppressible by dally^{sec.UAS.Tag:MYC}

(Takeo et al., 2005)

Scer\GAL4^A9, ptc^UAS.cJa has wing phenotype, non-suppressible by dally^{sec.UAS.Tag:MYC}

(Takeo et al., 2005)

Scer\GAL4^dpp.blk1, ptc^UAS.cJa has first posterior wing cell phenotype, non-suppressible by kn^UAS.cVa/Scer\GAL4^dpp.blk1

(Vervoort et al., 1999)

Scer\GAL4^en-e16E, ptc^UAS.cJa has first posterior wing cell phenotype, non-suppressible by Scer\GAL4^en-e16E/kn^UAS.cVa

(Vervoort et al., 1999)

Scer\GAL4^71B, ptc^UAS.cJa has wing vein phenotype, non-suppressible by hh^Mrtr1

(Johnson et al., 1995)

Scer\GAL4^71B, ptc^UAS.cJa has wing vein phenotype, non-suppressible by hh^Mrtr2

(Johnson et al., 1995)

Enhancer of

Statement

Reference

Scer\GAL4^Tab2-201Y/ptc^UAS.cJa is an enhancer of gamma Kenyon cell | somatic clone phenotype of Uvrag^LL03097

(Issman-Zecharya and Schuldiner, 2014)

ptc^UAS.cJa/Scer\GAL4^GMR71G10 is an enhancer of gamma Kenyon cell | somatic clone phenotype of Uvrag^LL03097

(Issman-Zecharya and Schuldiner, 2014)

ptc^UAS.cJa, Scer\GAL4^en-e16E is an enhancer of wing vein L3 phenotype of Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

ptc^UAS.cJa, Scer\GAL4^en-e16E is an enhancer of wing vein L4 phenotype of Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

ptc^UAS.cJa, Scer\GAL4^en-e16E is an enhancer of wing phenotype of Hsap\GLI3^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Aza-Blanc et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^NP6267/ptc^UAS.cJa is a suppressor of adult posterior midgut epithelium phenotype of Sulf1^KO.Cherry/Sulf1^ΔP1

(Takemura and Nakato, 2017)

NOT Suppressor of

Statement

Reference

ptc^UAS.cJa/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing phenotype of fu^{UAS.Tag:Palm(hGAP43),CFP}

(Claret et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The increased division of intestinal stem cells in the adult posterior midgut during normal homeostasis characteristic for Sulf1^KO;Cherry/Sulf1^ΔP1 mutants can be suppressed by expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood).

(Takemura and Nakato, 2017)

Co-expression of ptc^Scer\UAS.cJa significantly suppresses the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} over-expressed by Scer\GAL4^ppk.1.9.

(Im et al., 2015)

Expression of ptc^Scer\UAS.cJa under the control of either Scer\GAL4^Tab2-201Y or Scer\GAL4^GMR71G10 enhances the pruning defect seen in Uvrag^LL03097 mushroom body gamma neuron clones.

(Issman-Zecharya and Schuldiner, 2014)

Co-expression of ptc^Scer\UAS.cJa does not suppress the wing defects caused by expression of fu^{Scer\UAS.T:Hsap\Myr2,T:Avic\GFP-CFP} under the control of Scer\GAL4^Bx-MS1096.

(Claret et al., 2007)

When both ptc^Scer\UAS.cJa and dally^{sec.Scer\UAS.T:Hsap\MYC} are expressed under the control of Scer\GAL4^A9, the dally^{sec.Scer\UAS.T:Hsap\MYC} large body phenotype is suppressed, but the ptc^Scer\UAS.cJa wing phenotype remains.

(Takeo et al., 2005)

The addition of vn^L6 enhances the wing phenotype caused by ptc^Scer\UAS.cJa driven by Scer\GAL4^71B. The addition of vn^Scer\UAS.cSa suppresses the phenotype, restoring the anterior crossvein.

(Wessells et al., 1999)

ptc^Scer\UAS.cJa Scer\GAL4^71B flies carrying a relatively weakly expressing hh^Scer\UAS.cCa insertion line have wing discs that are indistinguishable from wild-type. ptc^Scer\UAS.cJa Scer\GAL4^71B flies carrying a more strongly expressing hh^Scer\UAS.cCa insertion line lack all veins in the anterior compartment, while vein 4 is restored. Ectopic expression of dpp^Scer\UAS.cCa in ptc^Scer\UAS.cJa Scer\GAL4^71B flies does not restore either presumptive veins 3 and 4 or the Sc25, L3 or ACV sensilla precursors. Most of the wing tissue, except vein 3 and its associated sensilla and vein 4, is recovered in ptc^Scer\UAS.cJa Scer\GAL4^MD-638 flies also carrying dpp^Scer\UAS.cCa. Flies expressing both ptc^Scer\UAS.cJa and hh^Scer\UAS.cCa using Scer\GAL4^MD-638 show recovery of the posterior compartment veins, ectopic induction of vein 3 and L3 sensilla and an enlargement of the region between veins 3 and 4.

(Mullor et al., 1997)

In a hh^Mrt background wing vein deletions are suppressed to mild vein fusions, hh^Mrtr1 and hh^Mrtr2 fail to suppress the wing vein deletion phenotype. In a Df(4)M101-62f or ci^Ce-1 the wing phenotype is enhanced.

(Johnson et al., 1995)

The wing vein deletions caused by expression of ptc^Scer\UAS.cJa under the control of Scer\GAL4^71B are suppressed to mild vein fusions in a hh^Mrt background, whereas hh^Mrtr1 and hh^Mrtr2 fail to suppress the phenotype. Df(4)M101-62f or ci^Ce-1 enhance the ptc^Scer\UAS.cJa; Scer\GAL4^71B wing phenotype.

(Johnson et al., 1995)

Xenogenetic Interactions

Statement

Reference

Expression of both Hsap\GLI3^{Scer\UAS.T:Hsap\MYC} and ptc^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E at 17^oC results in an enhancing, synergistic effect; the wings are significantly reduced in size. The central region of the wing, including veins 3 and 4 is absent. Higher levels of expression of both Hsap\GLI3^{Scer\UAS.T:Hsap\MYC} and ptc^Scer\UAS.cJa under the control of Scer\GAL4^en-e16E result in lethality.

(Aza-Blanc et al., 2000)

Complementation and Rescue Data

Rescues

Scer\GAL4^71B/ptc^UAS.cJa rescues ptc^Y442C.UAS

(Johnson et al., 2002)

Scer\GAL4^71B/ptc^UAS.cJa rescues ptc^1130X.UAS

(Johnson et al., 2000)

Partially rescues

ptc^UAS.cJa/Scer\GAL4^hs.PB partially rescues ptc¹⁰

(Lu et al., 2006)

ptc^UAS.cJa/Scer\GAL4^da.G32 partially rescues ptc¹⁶

(Johnson et al., 2000)

Comments

The ptc^{D584N.Scer\UAS} wing phenotype is suppressed by coexpression ptc^Scer\UAS.cJa (when driven by Scer\GAL4^71B).

(Johnson et al., 2002)

The addition of ptc^Scer\UAS.cJa (when driven by Scer\GAL4^da.G32) to ptc¹⁶ embryos complements the cuticle defects of these embryos - instead of 20% of embryos having a "ptc" phenotype, only 1% do.

(Johnson et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

5817

w^*; P{UAS-ptc.J}B1

44614

w^*; P{UAS-ptc.J}B3

44613

P{UAS-ptc.J}C, y¹ w^*