FlyBase Allele Report: Dmel\ptc[1130X.UAS]

General Information

Symbol

Dmel\ptc^1130X.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0124967

Feature type

allele

Associated gene

Dmel\ptc

Associated Insertion(s)

Carried in Construct

P{UAS-ptc.1130X}

Also Known As

UAS-Ptc^1130x, 1130X, UAS-Ptc^DN

Key Links

Transgenic product class

stop_gained

(Johnson et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Johnson et al., 2000)

Mutagen

in vitro construct

(Johnson et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-ptc.1130X}

Cytology

Description

UASt sequences drove expression of a mutated ptc - a stop codon and an XbalI site have been introduced after codon 1130.

(Johnson et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ptc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | early third instar larval stage | heat sensitive, with Scer\GAL4^ppk.1.9

(Im et al., 2015)

Phenotype Manifest In

double row of wing sensilla, with Scer\GAL4^71B

(Johnson et al., 2000)

double row of wing sensilla, with Scer\GAL4^71B, ptc¹⁰

(Johnson et al., 2000)

first basal wing cell, with Scer\GAL4^71B

(Johnson et al., 2002)

first posterior wing cell, with Scer\GAL4^71B

(Johnson et al., 2002)

wing, with Scer\GAL4^en-e16E

(Johnson et al., 2000)

wing | anterior, with Scer\GAL4^71B

(Johnson et al., 2002)

wing blade anterior compartment, with Scer\GAL4^71B

(Johnson et al., 2000)

wing sensillum | ectopic, with Scer\GAL4^71B

(Johnson et al., 2000)

wing vein | ectopic, with Scer\GAL4^71B

(Johnson et al., 2002, Johnson et al., 2000)

wing vein | ectopic, with Scer\GAL4^71B, ptc¹⁰

(Johnson et al., 2000)

wing vein L3, with Scer\GAL4^71B

(Johnson et al., 2000)

wing vein L3, with Scer\GAL4^71B, ptc¹⁰

(Johnson et al., 2000)

wing vein L4, with Scer\GAL4^71B

(Johnson et al., 2000)

wing vein L4, with Scer\GAL4^71B, ptc¹⁰

(Johnson et al., 2000)

Detailed Description

Statement

Reference

Compared to controls unresponsive to a 38[o]C probe (without pre-exposure to UV), early third instar larvae with expression of ptc^{1130X.Scer\UAS} driven by Scer\GAL4^ppk.1.9 show significant withdrawal responses (genetic allodynia).

(Im et al., 2015)

When ptc^{1130X.Scer\UAS} is driven by Scer\GAL4^71B, ectopic venation and expansion of the anterior region of the wing is seen. The intervein region between wing veins 3 and 4 is reduced.

(Johnson et al., 2002)

When ptc^{1130X.Scer\UAS} is driven by Scer\GAL4^71B the size of the anterior compartment of the wing is increased the posterior region is unaffected. The number of campaniform sensilla is increased, as is the length of the double row bristle region. There is slight disorganisation of the vein 3 region and proximal fusion of veins 3 and 4. The addition of heterozygous ptc¹⁰ enhances the wing phenotypes seen in ptc^{1130X.Scer\UAS}, Scer\GAL4^71B flies alone. There is increased venation in the vein 3 region and a larger row of bristles at the wing margin. When ptc^{1130X.Scer\UAS} is driven by Scer\GAL4^en-e16E, much of the wing pattern between veins 1 and 5 is deleted.

(Johnson et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ppk.1.9, ptc^1130X.UAS has abnormal pain response | early third instar larval stage | heat sensitive phenotype, suppressible by en^KK107874/Scer\GAL4^ppk.1.9

(Im et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.1.9, ptc^1130X.UAS has abnormal pain response | early third instar larval stage | heat sensitive phenotype, non-suppressible by TkR99D^GD375/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Scer\GAL4^ppk.1.9, ptc^1130X.UAS has abnormal pain response | early third instar larval stage | heat sensitive phenotype, non-suppressible by disp^GD2813/Scer\GAL4^ppk.1.9

(Im et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of TkR99D^GD375 does not suppress withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with expression of ptc^{1130X.Scer\UAS} driven by Scer\GAL4^ppk.1.9.

Co-expression of en^KK107874 suppresses the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with expression of ptc^{1130X.Scer\UAS} driven by Scer\GAL4^ppk.1.9.

Co-expression of disp^GD2813 does not significantly suppress the withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with ptc^{1130X.Scer\UAS} driven by Scer\GAL4^ppk.1.9.

(Im et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ptc^1130X.UAS is rescued by Scer\GAL4^71B/ptc^UAS.cJa

(Johnson et al., 2000)

Fails to rescue

ptc^1130X.UAS/Scer\GAL4^da.G32 fails to rescue ptc¹⁶

(Johnson et al., 2000)

Comments