FlyBase Allele Report: Dmel\TkR99D[GD375]

General Information

Symbol

Dmel\TkR99D^GD375

Species

D. melanogaster

Name

FlyBase ID

FBal0208720

Feature type

allele

Associated gene

Dmel\TkR99D

Associated Insertion(s)

Carried in Construct

P{GD375}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD375}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD375

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage | conditional, with Scer\GAL4^ppk.1.9

(Im et al., 2015)

abnormal pain response | early third instar larval stage | conditional, with Scer\GAL4^ppk.1.9

(Im et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

larval multidendritic class IV neuron | third instar larval stage | conditional, with Scer\GAL4^ppk.1.9

(Im et al., 2015)

Detailed Description

Statement

Reference

Expression of TkR99D^GD375 driven by Scer\GAL4^ppk.1.9 does not significantly alter baseline thermal nociception (uninjured early third instar larval responses to thermal probes at 45[o] or 48[o]C) compared to controls. 24 hours after UV-induced nociceptive sensitization, larvae with expression of TkR99D^GD375 driven by Scer\GAL4^ppk.1.9 show significant reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe) compared to controls.

Third instar larval fillets with expression of TkR99D^GD375 driven by Scer\GAL4^ppk.1.9 do not show a UV-induced increase in action potential firing rate at higher temperatures (36-39[o]C) in class IV neurons, as seen in controls.

(Im et al., 2015)

Adults expressing TkR99D^GD375 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

TkR99D^GD375/Scer\GAL4^ppk.1.9 is a non-suppressor of abnormal pain response | early third instar larval stage | heat sensitive phenotype of Scer\GAL4^ppk.1.9, ptc^1130X.UAS

(Im et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of TkR99D^GD375 does not suppress withdrawal responses to a 38[o]C probe (without pre-exposure to UV) seen in early third instar larvae with expression of ptc^{1130X.Scer\UAS} driven by Scer\GAL4^ppk.1.9.

(Im et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

TkR99D^GD375 is rescued by Scer\GAL4^ppk.1.9/TkR99D^UAS.EGFP

(Im et al., 2015)

Comments

Co-expression of TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} significantly rescues the reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe after UV-induced nociceptive sensitization) seen in early third instar larvae with TkR99D^GD375 driven by Scer\GAL4^ppk.1.9.

(Im et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v1372

w¹¹¹⁸; P{GD375}v1372

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Takr99D^GD375

TkR99D^GD375

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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