FlyBase Allele Report: Dmel\Lis-1[1.2.2]

General Information

Symbol

Dmel\Lis-1^1.2.2

Species

D. melanogaster

Name

FlyBase ID

FBal0120773

Feature type

allele

Associated gene

Dmel\Lis-1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lei and Warrior, 2000)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Lei and Warrior, 2000)

Amino acid replacement: W220term.

(Lei and Warrior, 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:16,182,899..16,182,899 [+]

Nucleotide change:

G16182899A

Reported nucleotide change:

G?A

Amino acid change:

W220term | Lis-1-PA; W220term | Lis-1-PB; W220term | Lis-1-PF; W220term | Lis-1-PG

Reported amino acid change:

W220term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Lei and Warrior, 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Lei and Warrior, 2000)

sterile (with Lis-1^k11702)

(Lei and Warrior, 2000)

Phenotype Manifest In

dorsal appendage (with Lis-1^k11702)

(Lei and Warrior, 2000)

Detailed Description

Statement

Reference

Eggs laid by Lis-1^1.2.2/Lis-1^k11702 mothers show defects in the location and morphology of the dorsal appendages.

(Lei and Warrior, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lis-1^1.2.2/Lis-1^k11702 has partially lethal - majority die phenotype, enhanceable by DCTN1-p150^Gl-1

(Lei and Warrior, 2000)

Phenotype Manifest In

Other

Statement

Reference

DCTN1-p150^Gl-1, Lis-1^1.2.2/Lis-1^k11702 has macrochaeta phenotype

(Lei and Warrior, 2000)

DCTN1-p150^Gl-1, Lis-1^1.2.2/Lis-1^k11702 has abdominal tergite phenotype

(Lei and Warrior, 2000)

DCTN1-p150^Gl-1, Lis-1^1.2.2/Lis-1^k11702 has microchaeta & abdomen | ventral phenotype

(Lei and Warrior, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The slight loss of viability phenotype seen in Lis-1^k11702/Lis-1^1.2.2, is enhanced by the addition of Gl¹. The adult escapers from this cross generally do not survive more than a few days. The addition of this Lis-1 transheterozygote combinations enhances the eye phenotype seen in Gl¹ flies. In addition scutellar bristles of escapers flies are reduced in size and frequently lost. Most adult escapers lack bristles on the ventral side of the abdomen, and in many animals abdominal tergites are completely absent in one or more segments.

(Lei and Warrior, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lis-1^1.2.2

Lis1^1.2.2

(Lei and Warrior, 2000)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close