Marked enok2 mutant female germline stem cells (GSCs) are present only 2 days after clone induction (ACI), but absent at 21 days ACI. Control clones are present at 21 days ACI. The mutant cells differentiate into marked mutant cysts. The cells in the mutant clones do not display signs of apoptosis.
Tracing of homozygous enok2 mutant cap cells in mosaic germaria for 21 days after eclosion reveals that the proportion of mutant clones drops dramatically over the time course, while the percentage the control ones remains steady.
Homozygotes tend to enter the wandering third instar larval stage at about 7 days after larval hatching (ALH) at 25oC while their heterozygous counterparts take only 3.5 to 4.0 days. Approximately half of the animals die before pupariation formation. Those that pupate do so at around 10 days ALH. Homozygotes never eclose as adults. Homozygous mutant clones exhibit defects in the mushroom body. Mushroom bodies contain only what appears to be a γ lobe and have a reduced cell number.