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General Information
Symbol
Dmel\EcRA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0122214
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-EcR-A, UAS-EcRA, UAS-EcR.A
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of a 3.26 kb EcoRI cDNA fragment containing the EcR-A isoform.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of EcRA.Scer\UAS in the developing wing under the control of Scer\GAL4Bx-MS1096 results in mild ectopic wing vein formation.

The number of niche cap cells and the number of germline cells containing a single spectrosome (SSCs) per germarium is increased in females expressing EcRA.Scer\UAS under the control of Scer\GAL4bab1-Pgal4-2.

Expression of EcRA.Scer\UAS under the control of Scer\GAL4Mef2.247.Switch results in an increase in the total amount of sleep during the day and at night compared to controls. Average sleep bout duration and daytime waking activity are also increased.

Expression of EcRA.Scer\UAS under the control of Scer\GAL4en-e16E results in some decrease in the hatching rate, but no lethality during metamorphosis; 78% of eggs laid eclose as adults. Prepupae expressing EcRA.Scer\UAS under the control of Scer\GAL4en-e16E have reduced tanning in the cuticle overlying posterior cells, resulting in puparia with a striped appearance.

Expression of EcRA.Scer\UAS when driven by Scer\GAL4Tab2-201Y does not result in any detectable abnormalities.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of EcRA.Scer\UAS enhances the G9aScer\UAS.cKa wing vein phenotype.

Co-expression of EcRA.Scer\UAS significantly suppresses the small wing phenotype caused by expression of pzgdsRNA.Scer\UAS under the control of Scer\GAL4en-e16E.

The addition of EcRA.Scer\UAS driven by Scer\GAL4Tab2-201Y partially rescues the gamma-lobe phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expressing EcRA.UAS under the control of Scer\GAL4CCAP.PP partially rescues both larval CCAP neuronal death and the reduced size of pupal legs and wings, while fully rescuing the head eversion phenotype induced by expression of EcRB1-ΔC655.W650A.UAS alone.

Co-expression of EcRA.Scer\UAS postpones larval lethality from the 1st instar to the 2nd/3rd instar transition, but results in little-to-no rescue of the Malpighian tubule phenotypes of EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4c42. Cuticle preparations of these larvae show molting defects.

Expression of EcRA.Scer\UAS under the control of Scer\GAL4ETH.Switch in the presence of RU486 partially rescues the lethality and failure of ecdysis phenotypes seen in larvae expressing EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4ETH.Switch in the presence of RU486.

The defects in midgut morphogenesis which are seen in embryos expressing EcRB1-ΔC655.W650A.Scer\UAS under the control of Scer\GAL4twi.PG are rescued by co-expression of EcRA.Scer\UAS.

The defects in tracheal morphogenesis which are seen in embryos expressing EcRB1-ΔC655.W650A.Scer\UAS under the control of Scer\GAL4btl.PS are rescued by co-expression of EcRA.Scer\UAS.

The embryonic defects caused by expression or EcRB1-ΔC655.W650A.Scer\UAS under the control of Scer\GAL469B are rescued by co-expression of EcRA.Scer\UAS.

Co-expression of EcRA.Scer\UAS rescues the lethality and eye defects caused by expression of EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4GMR.PF. Co-expression of EcRA.Scer\UAS rescues to normal viability and normal morphology animals expressing EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4Lsp2.PH or Scer\GAL4Eh.0.8. Co-expression of EcRA.Scer\UAS rescues to nearly complete viability animals expressing EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4dpp.blk1. Co-expression of EcRA.Scer\UAS rescues to normal viability and normal morphology animals expressing EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4Ser.PGF. Co-expression of EcRA.Scer\UAS partially rescues the glue secretion and puffing defects of animals expressing EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4Sgs3.PD. Co-expression of EcRA.Scer\UAS does not rescue the border cell migration defects, chorion fragility or fertility of females expressing EcRB1-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4slbo.2.6, and slightly enhances the phenotype, decreasing egg yield and increasing egg fragility.

The failure of pruning of the larval Tv-neurons seen in EcR31/EcR99 animals is slightly rescued by expression of EcRA.Scer\UAS under the control of Scer\GAL4Fmrf.PS.

EcRB2.Scer\UAS when driven by Scer\GAL4Tab2-201Y fails to rescue the Mushroom body γ neuron phenotypes.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
EcRA.Scer\UAS
EcRA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (23)