Insertion in between the AT and G of the translational start codon.
Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. Nonlinear mechanical amplification is significantly reduced compared to wild type.
BmcpBG02446 flies are highly sensitive to food deprivation compared to control flies, showing a 31.4% and 40.1% increase in median lethality for males and females respectively.
Developing and mature BmcpBG02446 flies have no obvious morphological developmental abnormalities. Mutant flies show a normal developmental time, on both a 5% yeast-sucrose low-calorie diet and on a 15% yeast-sucrose diet.
BmcpBG02446 flies have a 10-30% shorter median lifespan than control flies on two different types of severely calorie restricted diets (1% and 1.5% yeast-sucrose). On a 2% yeast-sucrose diet, BmcpBG02446 flies have up to 18% longer median lifespans than controls, and on a 5% yeast-sucrose diet, BmcpBG02446 flies show more than a 30% increase in median lifespan compared to controls. On a 10% or 15% yeast-sucrose diet, BmcpBG02446 flies have the same lifespan as control flies.
BmcpBG02446 females consistently lay fewer eggs than control females on a very-low-calorie diet (2% yeast-sucrose), a low-calorie diet (5% yeast-sucrose) or a high-calorie diet (15% yeast-sucrose), with the difference in number of eggs laid being the greatest on the low calorie diets.
On a 1% yeast-sucrose diet, both BmcpBG02446 and control flies lose weight rapidly, and after one week on the diet, BmcpBG02446 flies continue losing more weight than controls, up to the point at which the flies are presumably dying from starvation. On a 2% yeast-sucrose diet, both BmcpBG02446 and control flies maintain their baseline weight throughout the 3 week assay period. On a 5% yeast-sucrose diet, control flies gain slightly more weight than BmcpBG02446 flies. On a 10% or 15% yeast-sucrose diet, BmcpBG02446 flies gain less weight than control flies.
Mitochondria isolated from BmcpBG02446 flies (isolated from the head and thorax) show the same oxygen consumption ratio (state 3 respiration) and the same consumption ratio of oxygen after blocking ATP-synthase activity (state 4 respiration) as controls.
Normally fed BmcpBG02446 flies have an approximately 21% lower level of total sugars than control flies. BmcpBG02446 flies consume their triglyceride reserves at a much faster rate than control flies during starvation.
BmcpBG02446/Bmcp[+] is a non-enhancer of short lived phenotype of Hsap\HTTQ93.ex1.UAS, Scer\GAL4Eaat1.PR
BmcpBG02446/Bmcp[+] is a non-suppressor of short lived phenotype of Hsap\HTTQ93.ex1.UAS, Scer\GAL4Eaat1.PR
Heterozygosity for BmcpBG02446 does not affect the short adult life span phenotype of Hsap\HTTQ93.ex1p.Scer\UAS, Scer\GAL4Eaat1.PR flies.
BmcpBG02446 is partially rescued by Scer\GAL4elav-C155/BmcpUAS.Tag:FLAG
Precise excision of the insertion restores starvation resistance.