FlyBase Allele Report: Dmel\Su(var)2-10[537.UAS]

General Information

Symbol

Dmel\Su(var)2-10^537.UAS

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Betz

FlyBase ID

FBal0128056

Feature type

allele

Associated gene

Dmel\Su(var)2-10

Associated Insertion(s)

Carried in Construct

P{UAS-Su(var)2-10.537}

Also Known As

UAS-pias

Key Links

Transgenic product class

cDNA

(Betz et al., 2001)

wild_type

(Betz et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Betz et al., 2001)

wild_type

(Betz et al., 2001)

Mutagen

in vitro construct

(Betz et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Su(var)2-10.537}

Cytology

Description

UASt sequences drive expression of the Su(var)2-10 cDNA from EST clone AA803041. The cDNA encodes a protein 537 amino acids long.

(Betz et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Su(var)2-10

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | conditional, with Scer\GAL4^NP0001

(Cronin et al., 2009)

visible, with Scer\GAL4^ey.PH

(Betz et al., 2001)

wild-type, with Scer\GAL4^GMR.PF

(Betz et al., 2001)

Phenotype Manifest In

adult gut, with Scer\GAL4^NP0001

(Cronin et al., 2009)

eye, with Scer\GAL4^ey.PH

(Betz et al., 2001)

Detailed Description

Statement

Reference

Scer\GAL4^{Myo31DF-NP0001}-mediated expression of Su(var)2-10^537.Scer\UAS results in significantly increased survival following infection with S. marcescens compared to controls.

Scer\GAL4^{Myo31DF-NP0001}-mediated expression of Su(var)2-10^537.Scer\UAS results in a reduction of DNA synthesis in the gut epithelium after 5 days of infection with S. marcescens, compared to controls.

(Cronin et al., 2009)

When Su(var)2-10^537.Scer\UAS is driven by Scer\GAL4^GMR.PF, no obvious effect is seen on the eye. However when Su(var)2-10^537.Scer\UAS is driven by Scer\GAL4^ey.PH, eye size is severely reduced and the remaining small eye has a rough texture. This phenotype is dose dependant, leading to complete loss of eyes in many cases.

(Betz et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, Su(var)2-10^537.UAS has visible phenotype, enhanceable by Stat92E⁰⁶³⁴⁶

(Betz et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, Su(var)2-10^537.UAS has visible phenotype, suppressible by hop^Tum

(Betz et al., 2001)

Scer\GAL4^ey.PH, Su(var)2-10^537.UAS has visible phenotype, suppressible by Stat92E^hs.PY

(Betz et al., 2001)

Suppressor of

Statement

Reference

Su(var)2-10^537.UAS/Scer\GAL4^hs.PB is a suppressor of neoplasia phenotype of hop^Tum

(Betz et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, Su(var)2-10^537.UAS has eye phenotype, enhanceable by Stat92E⁰⁶³⁴⁶

(Betz et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, Su(var)2-10^537.UAS has eye phenotype, suppressible by Stat92E^hs.PY

(Betz et al., 2001)

Scer\GAL4^ey.PH, Su(var)2-10^537.UAS has eye phenotype, suppressible by hop^Tum

(Betz et al., 2001)

Other

Statement

Reference

Scer\GAL4^ey.PH, Stat92E⁰⁶³⁴⁶, Su(var)2-10^537.UAS has antenna | ectopic phenotype

(Betz et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of heat shock induced Stat92E^hs.PY significantly rescues the eye size and roughness seen in Su(var)2-10^537.Scer\UAS, Scer\GAL4^ey.PH flies. The addition of hop^Tum significantly rescues the eye size and roughness seen in Su(var)2-10^537.Scer\UAS, Scer\GAL4^ey.PH flies. The addition of Stat92E⁰⁶³⁴⁶ significantly enhances the eye size and roughness seen in Su(var)2-10^537.Scer\UAS, Scer\GAL4^ey.PH flies. The enhancement frequently goes further, progeny grow antenna in place of eyes.

(Betz et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Su(var)2-10^537.Scer\UAS

Su(var)2-10^537.UAS

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Betz

(Betz et al., 2001)

Secondary FlyBase IDs

References (4)

Report Sections

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