FlyBase Allele Report: Dmel\Cyfip[EP3267]

General Information

Symbol

Dmel\Cyfip^EP3267

Species

D. melanogaster

Name

FlyBase ID

FBal0130591

Feature type

allele

Associated gene

Dmel\Cyfip

Associated Insertion(s)

P{EP}Cyfip^EP3267

Carried in Construct

Also Known As

UAS-CYFIP, EP(3)3267

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Langmann et al., 2002, Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}Cyfip^EP3267

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:15,333,327..15,333,327 [+]

(Galy et al., 2011, Schenck et al., 2004, Schenck et al., 2003, Langmann et al., 2002, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage (with Cyfip^85.1)

(Galy et al., 2011)

partially lethal - majority live

(Galy et al., 2011, Schenck et al., 2003)

some die during pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

visible | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

Phenotype Manifest In

cone cell | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

cone cell | pupal stage (with Cyfip^85.1)

(Galy et al., 2011)

eye | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

interommatidial bristle | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

lens | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

ommatidium | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

photoreceptor | pupal stage (with Cyfip^85.1)

(Galy et al., 2011)

retina | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

rhabdomere | pharate adult stage (with Cyfip^85.1)

(Galy et al., 2011)

rhabdomere | pupal stage (with Cyfip^85.1)

(Galy et al., 2011)

Detailed Description

Statement

Reference

Homozygous Sra-1^EP3267 mutant adults display grossly normal eye morphology, but show subtle anomalies in ommatidia organisation with low penetrance.

Sra-1^EP3267/Sra-1^85.1 mutant pharate adults have rough eyes and display dark lenses in a speckled, random pattern indicating cone cell death. The ommatidia are occasionally misaligned or fused to their neighbours and some lack the typical smooth surface, instead showing crater-like intrusions indicative of lens material loss. Interommatidial bristles are often bent and appear soft and flat. Rhabdomeres appear bulky and never span the entire retina, either accumulating near the distal surface or underneath the retinal base (FM) in the lamina. The FM is mispositioned, leading to a markedly reduced retina depth. The rhabdomeres appear bulky and never span the entire retina, accumulating near the distal surface or underneath the FM in the lamina. The actin organisation of the FM is compromised and the polarised accumulation of dense cortical actin latices near the inner and outer lateral membrane of pigment cells is abolished.

Over 30% of Sra-1^EP3267/Sra-1^85.1 pupal photoreceptors possess ectopic membrane protrusions projecting from the stalks, a phenomenon never observed in wild type. Gaps also occur between the rhabdomeres and the cytoplasm and the rhabdomeres themselves comprise a massive amount of membrane that occupies an area that is twice as large as in controls, however the space occupied by the actin rich rhabdomere terminal web (RTW) is not proportionally increased. Actin fingers that normally extend from the sub-rhabdomeric space into the RTW are absent in Sra-1^EP3267/Sra-1^85.1 mutant cells but microvillar structure and stacking appear normal. Adherens junctions are correctly specified but structurally compromised. Defects in cone cell shape and configuration are also seen.

(Galy et al., 2011)

50% of animals die before eclosion. When Sra-1^EP3267 is driven in the eye by Scer\GAL4^elav-C155, no effects are seen on eye morphology.

(Schenck et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Cyfip^EP3267, Scer\GAL4^elav-C155 is a suppressor | partially of abnormal neuroanatomy phenotype of Fmr1^EP3517, Scer\GAL4^elav-C155

(Schenck et al., 2004)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Cyfip^EP3267, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Cyfip^EP3267, Scer\GAL4^GMR.PF is a non-enhancer of ommatidium phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Suppressor of

Statement

Reference

Cyfip^EP3267, Scer\GAL4^elav-C155 is a suppressor | partially of neuromuscular junction & synapse phenotype of Fmr1^EP3517, Scer\GAL4^elav-C155

(Schenck et al., 2004)

Cyfip^EP3267, Scer\GAL4^GMR.PF is a suppressor | partially of ommatidium phenotype of Rac1^V12.UAS, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Cyfip^EP3267, Scer\GAL4^GMR.PF is a suppressor | partially of eye | posterior phenotype of Rac1^V12.UAS, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Cyfip^EP3267, Scer\GAL4^GMR.PF is a suppressor | partially of ommatidium phenotype of Fmr1^EP3517, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Cyfip^EP3267, Scer\GAL4^elav-C155 is a suppressor | partially of synapse phenotype of Fmr1^EP3517, Scer\GAL4^elav-C155

(Schenck et al., 2003)

Cyfip^EP3267, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Fmr1^EP3517, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Cyfip^EP3267, Scer\GAL4^elav-C155 is a suppressor of larval intersegmental nerve phenotype of Rac1^V12.UAS, Scer\GAL4^elav-C155

(Schenck et al., 2003)

NOT Suppressor of

Statement

Reference

Cyfip^EP3267, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Cyfip^EP3267, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Schenck et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced synaptic length seen at the neuromuscular junction in larvae expressing Fmr1^EP3517 under the control of Scer\GAL4^elav-C155 is partially suppressed by co-expression of Sra-1^EP3267.

(Schenck et al., 2004)

The combination of Sra-1^EP3267 and Rac1^V12.Scer\UAS driven by Scer\GAL4^GMR.PF has a weaker eye phenotype than Rac1^V12.Scer\UAS (driven by Scer\GAL4^GMR.PF) alone. The combination of Sra-1^EP3267 and Rac1^V12.Scer\UAS driven by Scer\GAL4^elav-C155 has a ISNb stalling phenotype (only 17% exhibit the phenotype compared to 29%) than Rac1^V12.Scer\UAS (driven by Scer\GAL4^elav-C155) alone.

(Schenck et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Cyfip^EP3267 is partially rescued by Cyfip^EP3267/Scer\GAL4^GMR.PF

(Galy et al., 2011)

Partially rescues

Cyfip^EP3267/Scer\GAL4^GMR.PF partially rescues Cyfip^EP3267

(Galy et al., 2011)

Comments

Expression of Sra-1^EP3267 under the control of Scer\GAL4^GMR.PF fully rescues the eye morphology defects seen in Sra-1^EP3267/Sra-1^85.1 mutant pharate adults. The lethality associated with Sra-1^EP3267/Sra-1^85.1 is not rescued.

Expression of Sra-1^EP3267 under the control of Scer\GAL4^lz-gal4 fully rescues the retinal fenestrated membrane integrity defects seen in Sra-1^EP3267/Sra-1^85.1 mutant pharate adults. As result photoreceptors are kept in place and no longer fall into the lamina. The dark necrotic lenses seen in mutant flies are also absent. Some bulky rhabdomeres are still seen, but fewer than are seen in the mutant alone.

(Galy et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CYFIP^EP3267

(Qurashi et al., 2007)

Cyfip^EP3267

Sra-1^EP3267

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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