FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\blanksKG00804
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General Information
Symbol
Dmel\blanksKG00804
Species
D. melanogaster
Name
FlyBase ID
FBal0134398
Feature type
allele
Associated gene
Dmel\blanks
Associated Insertion(s)
P{SUPor-P}blanksKG00804
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Sanders and Smith, 2011)
Mutagen
P-element activity
(Gene Disruption Project members, 2001-)
Progenitor genotype
Associated Insertion(s)
P{SUPor-P}blanksKG00804
Cytology
Description

Insertion 29bp downstream of the predicted transcription start site.

(Gerbasi et al., 2011)

Insertion in 5' UTR of blanks, 70 bp upstream of the coding region.

(Sanders and Smith, 2011)
Allele components
Component
Use(s)
Inserted element
P{SUPor-P}
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous males lack mature sperm in the seminal vesicle. Spermatocyte development occurs normally through all the mitotic divisions and both rounds of meiosis. Nuclear elongation and axoneme development are grossly normal. However, syncytial spermatid bundles fail to fully individualise, resulting in non-motile, decomposing spermatid cysts. The actin rich individualization complexes show asynchronous migration during individualization.

      (Gerbasi et al., 2011)

      blanksKG00804 homozygous females crossed to wild type males produce a similar number of offspring as controls. However, no progeny are produced when homozygous blanksKG00804 males, or blanksKG00804/Df(3L)Exel6105 males are crossed to wild type females.

      No mature sperm are present in seminal vesicles from blanksKG00804 homozygous males.

      Early spermatids from blanksKG00804 mutants are indistinguishable from wild type. However, defects are seen in spermatid individualization: mutant testes contain syncitial spermatid bundles with irregular bulges and 'lumps' along the length of the tail clusters. During the elongation phase, blanksKG00804 spermatid nuclei alignment is disrupted, and the nuclei are scattered and out of register. DNA condensation appears unaffected, as judged by P{ProtA-EGFP.Z} expression. The initial formation of the individualization complex (IC) also appears normal in the mutant. However, the ICs fail to translocate in a coordinated fashion and they become misaligned with stalled bundle migrations.

      (Sanders and Smith, 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      blanksKG00804 is a non-suppressor of abnormal eye color phenotype of Scer\GAL4GMR.PF, wRNAi.UAS.cKa

      (Sanders and Smith, 2011)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The eye colour of wdsRNA.Scer\UAS.cKa, Scer\GAL4GMR.PF flies remains orange in a blanksKG00804 background.

      (Sanders and Smith, 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      blanksKG00804 is rescued by blanks+t6

      (Sanders and Smith, 2011)

      blanksKG00804 is rescued by blanksGFP

      (Sanders and Smith, 2011)

      blanksKG00804 is rescued by blanksdsRBD2

      (Sanders and Smith, 2011)
      Not rescued by

      blanksKG00804 is not rescued by blanksdsRBD1

      (Sanders and Smith, 2011)

      blanksKG00804 is not rescued by blanksdsRBD1+2

      (Sanders and Smith, 2011)
      Comments

      The sterility of homozygous blanksKG00804 males is rescued by a blanks+t6 transgene.

      Mature sperm are present in seminal vesicles from blanksKG00804 homozygous males rescued with blanks+t6.

      blanks+t6 rescues the individualization complex defect seen in blanksKG00804 mutants.

      (Sanders and Smith, 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Precise excision of the insertion reverts the male sterile phenotype.

      (Gerbasi et al., 2011)

      Precise excision revertants of P{SUPor-P}blanksKG00804 are fertile, demonstrating P{SUPor-P}blanksKG00804 is responsible for the fertility defect.

      (Sanders and Smith, 2011)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      CG10630KG00804
      (Sanders and Smith, 2011)
      blanksKG00084
      (Fingerhut et al., 2019)
      blanksKG00804
      (Gerbasi et al., 2011)
      lump1
      (Sanders and Smith, 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)