FlyBase Allele Report: Dmel\brat[UAS.cFa]

General Information

Symbol

Dmel\brat^UAS.cFa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Frank

FlyBase ID

FBal0135749

Feature type

allele

Associated gene

Dmel\brat

Associated Insertion(s)

Carried in Construct

P{UAS-brat.F}

Key Links

Transgenic product class

wild_type

(Frank et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Frank et al., 2002)

Mutagen

in vitro construct

(Frank et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-brat.F}

Cytology

Description

UASt sequences drive expression of brat.

(Frank et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

brat

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | somatic clone, with Scer\GAL4^Act5C.PP

(Frank et al., 2002)

increased cell size, with Scer\GAL4^dpp.blk1

(Frank et al., 2002)

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

(Frank et al., 2002)

visible, with Scer\GAL4^ey.PH

(Frank et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Frank et al., 2002)

first posterior wing cell, with Scer\GAL4^dpp.blk1

(Frank et al., 2002)

mitotic cell cycle | somatic clone, with Scer\GAL4^Act5C.PP

(Frank et al., 2002)

Detailed Description

Statement

Reference

When brat^Scer\UAS.cFa is driven by Scer\GAL4^ey.PH in the developing eye, a dramatic decrease in eye size. When brat^Scer\UAS.cFa is driven by Scer\GAL4^dpp.blk1, a 36% decrease in the size of the intervein region between LIII and LIV is seen compared to wild-type. However cell size appears to increase. When brat^Scer\UAS.cFa clones are made driven by Scer\GAL4^Act5C.PP, the clones are seen to be smaller in size than their wild-type counterparts, but the cell size within these clones is slightly larger. Cells within clones have 50% longer doubling times than control cells.

(Frank et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PP, brat^UAS.cFa has increased cell size | somatic clone phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP

(Frank et al., 2002)

Scer\GAL4^Act5C.PP, brat^UAS.cFa has decreased cell number | somatic clone phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP

(Frank et al., 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

When BacA\p35^Scer\UAS.cHa is added to brat^Scer\UAS.cFa somatic clones (driven by Scer\GAL4^Act5C.PP) cells within the clones become larger, but the overall clone size is reduced.

(Frank et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

brat^Scer\UAS.cFa

brat^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Frank

(Frank et al., 2002)

Secondary FlyBase IDs

References (1)

Report Sections

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