FlyBase Allele Report: Dmel\Pp2B-14D[act.UAS]

General Information

Symbol

Dmel\Pp2B-14D^act.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0137391

Feature type

allele

Associated gene

Dmel\Pp2B-14D

Associated Insertion(s)

Carried in Construct

P{UAS-Pp2B-14D.act}

Also Known As

UAS-Pp2B-14D^Act

Key Links

Transgenic product class

stop_gained

(Sullivan and Rubin, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Sullivan and Rubin, 2002)

Mutagen

in vitro construct

(Sullivan and Rubin, 2002)

Progenitor genotype

Carried in construct

P{UAS-Pp2B-14D.act}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of Pp2B-14D that has a stop codon introduced at residue 456.

(Sullivan and Rubin, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pp2B-14D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2010)

abnormal size, with Scer\GAL4^en-e16E

(Takeo et al., 2010)

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2010)

lethal, with Scer\GAL4^how-24B

(Takeo et al., 2006)

visible, with Scer\GAL4^en-e16E

(Sullivan and Rubin, 2002)

visible, with Scer\GAL4^hs.2sev

(Sullivan and Rubin, 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Wang et al., 2008)

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Sullivan and Rubin, 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Takeo et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Takeo et al., 2006)

photoreceptor cell R3, with Scer\GAL4^hs.2sev

(Sullivan and Rubin, 2002)

photoreceptor cell R4, with Scer\GAL4^hs.2sev

(Sullivan and Rubin, 2002)

photoreceptor cell R7, with Scer\GAL4^hs.2sev

(Sullivan and Rubin, 2002)

wing, with Scer\GAL4^en-e16E

(Takeo et al., 2006)

wing blade, with Scer\GAL4^en-e16E

(Takeo et al., 2010)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Sullivan and Rubin, 2002)

wing vein, with Scer\GAL4^en-e16E

(Takeo et al., 2010, Takeo et al., 2006, Sullivan and Rubin, 2002)

Detailed Description

Statement

Reference

Male flies mis-expressing the constitutively active Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^elav-C155 and Scer\GAL80^ts.αTub84B at 30[o]C for 2 days (to allow gene expression) and then at 19[o]C exhibit no difference in sleep patterns compared to controls.

(Tomita et al., 2011)

Overexpression of Pp2B-14D^act.Scer\UAS driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} induces female sterility with meiotic defects.

Overexpression of Pp2B-14D^act.Scer\UAS in the developing wing driven by Scer\GAL4^en-e16E causes loss of wing veins and reduction in wing size. Co-expression of Pp2B-14D^{DN.Scer\UAS.P\T} with Pp2B-14D^act.Scer\UAS driven by Scer\GAL4^en-e16E suppresses the Pp2B-14D^act.Scer\UAS-induced phenotypes.

(Takeo et al., 2010)

Eye-specific expression of Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^GMR.PF promotes a rough eye phenotype in wild-type flies.

(Wang et al., 2008)

Flies that express Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^GMR.PF show a mild rough eye phenotype.

Expression of Pp2B-14D^act.Scer\UAS in the posterior compartment of developing imaginal discs, driven by Scer\GAL4^en-e16E, results in the loss of wing veins and reduction of wing size.

(Takeo et al., 2006)

Expression of Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^en-e16E results in a truncation of wing veins and a decrease in size of the posterior compartment of the wing. Expression of Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^hs.2sev results in a decrease in the number of photoreceptor cells per ommatidium (penetrance of the phenotype is 8%). The R7 cell is usually missing, but sometimes R3 or R4 are missing.

(Sullivan and Rubin, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pp2B-14D^act.UAS, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, suppressible | partially by Df(2R)ST1/+

(Takeo et al., 2010)

Pp2B-14D^act.UAS, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, suppressible | partially by CanB2^EP774/CanB2[+]

(Takeo et al., 2010)

Pp2B-14D^act.UAS, Scer\GAL4^how-24B has lethal phenotype, suppressible by sra^UAS.cEa, Scer\GAL4^how-24B

(Takeo et al., 2006)

NOT suppressed by

Statement

Reference

Pp2B-14D^act.UAS, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, non-suppressible by +/Df(2R)6149

(Takeo et al., 2010)

Pp2B-14D^act.UAS, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, non-suppressible by Df(2R)Exel6055/+

(Takeo et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pp2B-14D^act.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Crtc^UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2008)

Suppressed by

Statement

Reference

Pp2B-14D^act.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Crtc^25-3

(Wang et al., 2008)

Pp2B-14D^act.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by sra^UAS.cEa, Scer\GAL4^GMR.PF

(Takeo et al., 2006)

Pp2B-14D^act.UAS, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, suppressible by sra^UAS.cEa, Scer\GAL4^GMR.PF

(Takeo et al., 2006)

Pp2B-14D^act.UAS, Scer\GAL4^en-e16E has wing vein phenotype, suppressible by sra^UAS.cEa, Scer\GAL4^en-e16E

(Takeo et al., 2006)

Pp2B-14D^act.UAS, Scer\GAL4^en-e16E has wing phenotype, suppressible by sra^UAS.cEa, Scer\GAL4^en-e16E

(Takeo et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2R)ST1/+ partially suppresses the female sterility associated with the overexpression of Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

CanB2^EP774/+ partially suppresses the female sterility associated with the overexpression of Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Takeo et al., 2010)

Eye-specific expression of Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^GMR.PF fails to promote a rough eye phenotype in TORC^25-3 mutant flies.

Over-expression of TORC^Scer\UAS.cWa strongly potentiates the effects of Pp2B-14D^act.Scer\UAS expression on eye morphology.

(Wang et al., 2008)

The rough eye phenotype seen in flies that express Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^GMR.PF is completely suppressed by coexpression of sra^Scer\UAS.cEa. Additionally, the wing phenotypes seen in flies expressing Pp2B-14D^act.Scer\UAS under the control of Scer\GAL4^en-e16E are completely suppressed by sra^Scer\UAS.cEa coexpression.

(Takeo et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pp2B-14D^act.Scer\UAS

Pp2B-14D^act.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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