FlyBase Allele Report: Dmel\Lkb1[UASp.GFP]

General Information

Symbol

Dmel\Lkb1^UASp.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0141046

Feature type

allele

Associated gene

Dmel\Lkb1

Associated Insertion(s)

Carried in Construct

P{UASp-GFP-Lkb1.wt}

Also Known As

UAS-GFP-LKB1

Key Links

Transgenic product class

genomic_DNA

(Martin and St. Johnston, 2003)

wild_type

(Martin and St. Johnston, 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Martin and St. Johnston, 2003)

wild_type

(Martin and St. Johnston, 2003)

Mutagen

in vitro construct

(Martin and St. Johnston, 2003)

Progenitor genotype

Carried in construct

P{UASp-GFP-Lkb1.wt}

Cytology

Description

UASp regulatory sequences control expression of a 3.6kb genomic fragment that contains the wild-type Lkb1 transcription unit. A GFP tag has been inserted at the N-terminal end of the Lkb1 open reading frame.

(Martin and St. Johnston, 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Lkb1

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Phenotype Manifest In

wing, with Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Detailed Description

Statement

Reference

The expression of Lkb1^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^ap-md544 leads to adult wings presenting an upward curvature.

(O'Farrell et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lkb1^UASp.GFP, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Wdfy2^{R323A.UAS.mCherry}, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Enhancer of

Statement

Reference

Lkb1^UASp.GFP, Scer\GAL4^ap-md544 is an enhancer of visible phenotype of Scer\GAL4^ap-md544, Wdfy2^{R323A.UAS.mCherry}

(O'Farrell et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lkb1^UASp.GFP, Scer\GAL4^ap-md544 has wing phenotype, enhanceable by Wdfy2^{R323A.UAS.mCherry}, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Enhancer of

Statement

Reference

Lkb1^UASp.GFP, Scer\GAL4^ap-md544 is an enhancer of wing phenotype of Scer\GAL4^ap-md544, Wdfy2^{R323A.UAS.mCherry}

(O'Farrell et al., 2017)

Suppressor of

Statement

Reference

Lkb1^UASp.GFP/Scer\GAL4^{VP16.mat.αTub67C} is a suppressor | partially of oocyte phenotype of par-1^k06323/par-1^W3

(Martin and St Johnston, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Lkb1^{Scer\UAS.P\T.T:Avic\GFP} and Wdfy2^{R323A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ap-md544 enhances the wing defects observed upon either single expression.

(O'Farrell et al., 2017)

lkb1^{Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} partially rescues the loss of anterior-posterior polarity seen in the oocytes of par-1^k06323/par-1^W3 animals.

(Martin and St Johnston, 2003)

Loss of oocyte polarity in par-1^k06323/par-1^W3 is partially suppressed by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}; lkb1^{Scer\UAS.P\T.T:Avic\GFP}.

(Martin and St. Johnston, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Lkb1^UASp.GFP/Scer\GAL4^{VP16.mat.αTub67C} rescues Lkb1^4A4-2

(Martin and St Johnston, 2003)

Lkb1^UASp.GFP/Scer\GAL4^{VP16.mat.αTub67C} rescues Lkb1^4A4-2

(Martin and St. Johnston, 2003)

Scer\GAL4^arm.PS/Lkb1^UASp.GFP rescues Lkb1^4A4-2

(Martin and St. Johnston, 2003)

Partially rescues

Scer\GAL4^arm.PS/Lkb1^UASp.GFP partially rescues Lkb1^4B1-11/Lkb1^4A4-2

(Martin and St. Johnston, 2003)

Comments

lkb1^{Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the loss of anterior-posterior polarity seen in the lkb1^4A4-2 germ line clone oocytes.

(Martin and St Johnston, 2003)

Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}; lkb1^{Scer\UAS.P\T.T:Avic\GFP} completely rescues the loss of polarity in lkb1^4A4-2 germ line clone oocytes. lkb1^{Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^arm.PS rescues the lethality due to lkb1^4A4-2/lkb1^4B1-11. The ovaries of the resulting flies have largely normal organisation of the follicular epithelium and normal polarity in approximately 50% of their oocytes.

(Martin and St. Johnston, 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80972

w^*; P{UASp-GFP-Lkb1.wt}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Lkb1^{Scer\UAS.P\T.T:Avic\GFP}

Lkb1^UASp.GFP

lkb1^{Scer\UAS.P\T.T:Avic\GFP}

lkb1^{Ser\UAS.P\T.T:Avic\GFP}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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