In the dark in the presence of a virgin female, PoxnΔM22-B5/PoxnΔM22-B5 males rescued by simultaneously carrying both PoxnΔXBs and PoxnΔPBs scan for the female by spreading their wings and scanning in a zigzag course, as do wild-type males in this courtship assay.
Only half of PoxnΔM22-B5/PoxnΔM22-B5 males rescued by simultaneously carrying both PoxnΔXBs and PoxnΔPBs initiate courtship in the dark, but their latency does not differ significantly from that of wild type. After courtship initiation the mutant males court females with the same vigor as do wild-type males.
PoxnΔM22-B5/PoxnΔM22-B5 males rescued by simultaneously carrying both PoxnΔXBs and PoxnΔPBs court with the same intensity as wild-type males in daylight.
PoxnΔM22-B5/PoxnΔM22-B5 males rescued by simultaneously carrying both PoxnΔXBs and PoxnΔPBs show no preference for courtship initiation towards decapitated females or males in the dark, with only half of them initiating courtship. These males court decapitated females and males with indistinguishable high courtship vigor indexes.
PoxnΔM22-B5/PoxnΔM22-B5 males rescued by simultaneously carrying both PoxnΔXBs and PoxnΔPBs show a significantly higher courtship vigor index towards intact or dewinged males in the dark compared to control wild-type males facing intact males.
PoxnΔM22-B5/PoxnΔM22-B5 males rescued by simultaneously carrying both PoxnΔXBs and PoxnΔPBs are clearly able to distinguish between decapitated males and females in daylight in a courtship assay.
PoxnΔM22-B5/PoxnΔM22-B5 males rescued by simultaneously carrying both PoxnΔXBs and PoxnΔPBs show courtship chains when groups of mature, but sexually naive, males are placed together in a petri dish.
Orco2, PoxnΔM22-B5, PoxnΔPBs, PoxnΔXBs has abnormal courtship behavior | male phenotype
Groups of naive w1118 ; "Poxn-pRes" (PoxnΔXBs, PoxnΔM22-B5/PoxnΔM22-B5, PoxnΔPBs) males (which carry two copies of w+mW.hs - one in each of the P{Poxn.ΔXBs} and P{Poxn.ΔPBs} constructs) show strongly enhanced chaining behaviour compared to "Poxn-pRes" males with a w[+] genomic background, suggesting that the reduced sexual discrimination of "Poxn-pRes" males is independent of the reduced sexual discrimination seen in w1118 males carrying a mini-w gene.
PoxnΔXBs; PoxnΔM22-B5/PoxnΔM22-B5 PoxnΔPBs; Or83b2 males show virtually no courtship activities in the dark. Only 10% of these males initiate courtship with a long latency, and their courtship is limited to a few seconds of wing extension.
In daylight, PoxnΔXBs; PoxnΔM22-B5/PoxnΔM22-B5 PoxnΔPBs; Or83b2 males initiate courtship fairly reliably and rather quickly compared to control males. However, they show a strongly reduced courtship vigor index compared to controls.
The fraction of PoxnΔXBs; PoxnΔM22-B5/PoxnΔM22-B5 PoxnΔPBs; Or83b2 males initiating courtship of decapitated animals in the dark is marginal, but those males that do initiate courtship court decapitated flies vigorously.
PoxnΔXBs partially rescues PoxnΔM22-B5
PoxnΔXBs partially rescues PoxnΔM22-B5
PoxnΔXBs does not completely rescue the leg/antenna segmentation phenotype of PoxnΔM22-B5 animals.
The defects in taste bristles on the leg, labellum and anterior wing margin seen in PoxnΔM22-B5 flies are not rescued by PoxnΔXBs. The leg and antenna segmentation defects seen in PoxnΔM22-B5 flies are partially rescued by PoxnΔXBs. The defects in the posterior lobe and penis seen in PoxnΔM22-B5 males are rescued by PoxnΔXBs and male fertility is partially rescued. PoxnΔXBs does not rescue the flying capability of PoxnΔM22-B5 flies. PoxnΔXBs rescues the courting defects in daylight conditions of PoxnΔM22-B5 males and the rescued males show some courtship under red light conditions.
