In the dark in the presence of a virgin female, Poxn^ΔM22-B5/Poxn^ΔM22-B5 males rescued by simultaneously carrying both Poxn^ΔXBs and Poxn^ΔPBs scan for the female by spreading their wings and scanning in a zigzag course, as do wild-type males in this courtship assay.

Only half of Poxn^ΔM22-B5/Poxn^ΔM22-B5 males rescued by simultaneously carrying both Poxn^ΔXBs and Poxn^ΔPBs initiate courtship in the dark, but their latency does not differ significantly from that of wild type. After courtship initiation the mutant males court females with the same vigor as do wild-type males.

Poxn^ΔM22-B5/Poxn^ΔM22-B5 males rescued by simultaneously carrying both Poxn^ΔXBs and Poxn^ΔPBs court with the same intensity as wild-type males in daylight.

Poxn^ΔM22-B5/Poxn^ΔM22-B5 males rescued by simultaneously carrying both Poxn^ΔXBs and Poxn^ΔPBs show no preference for courtship initiation towards decapitated females or males in the dark, with only half of them initiating courtship. These males court decapitated females and males with indistinguishable high courtship vigor indexes.

Poxn^ΔM22-B5/Poxn^ΔM22-B5 males rescued by simultaneously carrying both Poxn^ΔXBs and Poxn^ΔPBs show a significantly higher courtship vigor index towards intact or dewinged males in the dark compared to control wild-type males facing intact males.

Poxn^ΔM22-B5/Poxn^ΔM22-B5 males rescued by simultaneously carrying both Poxn^ΔXBs and Poxn^ΔPBs are clearly able to distinguish between decapitated males and females in daylight in a courtship assay.

Poxn^ΔM22-B5/Poxn^ΔM22-B5 males rescued by simultaneously carrying both Poxn^ΔXBs and Poxn^ΔPBs show courtship chains when groups of mature, but sexually naive, males are placed together in a petri dish.

Orco², Poxn^ΔM22-B5, Poxn^ΔPBs, Poxn^ΔXBs has abnormal courtship behavior | male phenotype