UAS regulatory sequences drive expression of Syt1, which is tagged at the C-terminal end with a Tag:CALI(4C) tag.
Vesicles at synapses in which Syt1Scer\UAS.T:Zzzz\4C (under the control of Scer\GAL4elav.PLu) is specifically photoactivated in the endocytic phase of the vesicle cycle are significantly larger than controls. Dramatically enlarged vesicular structures that often exceed 100nm are observed throughout the vesicle pool.