FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\p53D259H.UAS
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General Information
Symbol
Dmel\p53D259H.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0146961
Feature type
allele
Associated gene
Dmel\p53
Associated Insertion(s)
Carried in Construct
P{UAS-p53.D259H}
Key Links
Transgenic product class
dominant_negative_variant
(Peters et al., 2002)
missense_variant
(Peters et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Peters et al., 2002)
missense_variant
(Peters et al., 2002)
Mutagen
in vitro construct
(Peters et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-p53.D259H}
Cytology
Description

UAS regulatory sequences drive expression of a dominant-negative form of p53 (carries the amino acid replacement D259H).

(Peters et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
p53
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Irradiated eye discs from larvae expressing p53D259H.Scer\UAS under the control of Scer\GAL4GMR.PF have few apoptotic cells posterior to the morphogenetic furrow (where p53D259H.Scer\UAS is expressed) and only have abundant apoptotic cells anterior to the furrow (wild-type irradiated disc have an increased number of apoptotic cells both anterior and posterior to the furrow).

      (Peters et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Apoptotic cell elimination induced by nabEP642 expression in wing imaginal disc clones (under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) is not affected by co-expression of p53D259H.Scer\UAS.

      Clones of imaginal wing disc cells co-expressing both nabEP642 and p53D259H.Scer\UAS (under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) in a Df(3L)H99 heterozygous background exhibit non-autonomous tissue overgrowth.

      (Suissa et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      p53D259H.Scer\UAS
      p53D259H.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)