FlyBase Allele Report: Dmel\neur[ΔRING.UAS.EGFP]

General Information

Symbol

Dmel\neur^{ΔRING.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0146982

Feature type

allele

Associated gene

Dmel\neur

Associated Insertion(s)

Carried in Construct

P{UAS-neur.ΔRING::EGFP}

Also Known As

UAS-neurΔRING-GFP

Key Links

Transgenic product class

inframe_deletion

(Pavlopoulos et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Pavlopoulos et al., 2001)

Mutagen

in vitro construct

(Pavlopoulos et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-neur.ΔRING::EGFP}

Cytology

Description

Expression of the first 623 amino acids of neur fused in frame with EGFP is under the control of UASt sequences.

(Pavlopoulos et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

neur

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 8

modeled by Hsap\LRRK2^UAS.cVa

(Imai et al., 2015)

modeled by Hsap\LRRK2^RG.UAS

(Imai et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^c747

(Pavlopoulos et al., 2008)

abnormal memory, with Scer\GAL4^Mef2.247

(Pavlopoulos et al., 2008)

visible | adult stage, with Scer\GAL4^dpp.PU

(Imai et al., 2015)

Phenotype Manifest In

wing margin, with Scer\GAL4^dpp.PU

(Imai et al., 2015)

Detailed Description

Statement

Reference

Expression of neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dpp.PU results in notching phenotype in the adult wing margin.

(Imai et al., 2015)

Expression of neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^c747 does not affect learning. 260-minute and 24-hour memories are significantly higher in neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP}-expressing flies than in controls. Enhanced memory is also observed when neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^Mef2.247 but not Scer\GAL4^c739 or Scer\GAL4^H24. These flies respond normally to stimuli and mushroom body morphology is indistinguishable from controls.

(Pavlopoulos et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

neur^{ΔRING.UAS.EGFP} has visible | adult stage phenotype, enhanceable by Scer\GAL4^dpp.PU/Neurl4^EY12221

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has visible | adult stage phenotype, enhanceable by Scer\GAL4^dpp.PU/Hsap\LRRK2^GS.UAS

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has visible | adult stage phenotype, enhanceable by Scer\GAL4^dpp.PU/Hsap\LRRK2^KD.UAS

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has visible | adult stage phenotype, enhanceable by Scer\GAL4^dpp.PU/HERC2^G17171

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has visible | adult stage phenotype, enhanceable by Hsap\LRRK2^UAS.cVa/Scer\GAL4^dpp.PU

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has visible | adult stage phenotype, enhanceable by Hsap\LRRK2^RG.UAS/Scer\GAL4^dpp.PU

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has visible | adult stage phenotype, enhanceable by Scer\GAL4^dpp.PU/Hsap\LRRK2^TN.UAS

(Imai et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, enhanceable by Scer\GAL4^dpp.PU/Neurl4^EY12221

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, enhanceable by Scer\GAL4^dpp.PU/Hsap\LRRK2^GS.UAS

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, enhanceable by Scer\GAL4^dpp.PU/Hsap\LRRK2^KD.UAS

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, enhanceable by Scer\GAL4^dpp.PU/HERC2^G17171

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, enhanceable by Hsap\LRRK2^UAS.cVa/Scer\GAL4^dpp.PU

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, enhanceable by Hsap\LRRK2^RG.UAS/Scer\GAL4^dpp.PU

(Imai et al., 2015)

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, enhanceable by Scer\GAL4^dpp.PU/Hsap\LRRK2^TN.UAS

(Imai et al., 2015)

Suppressed by

Statement

Reference

neur^{ΔRING.UAS.EGFP} has wing margin phenotype, suppressible | partially by Lrrk^e03680/Lrrk^e03680

(Imai et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The wing notching phenotype characteristic for adults expressing neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dpp.PU is exacerbated by co-expression of either Neurl4^EY12221 or HERC2^G17171 and partially suppressed by combination with Lrrk^e03680 in homozygous state.

(Imai et al., 2015)

Xenogenetic Interactions

Statement

Reference

The wing notching phenotype characteristic for adults expressing neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dpp.PU is exacerbated by co-expression of any of the following: Hsap\LRRK2^Scer\UAS.cVa, Hsap\LRRK2^RG.Scer\UAS, Hsap\LRRK2^TN.Scer\UAS, Hsap\LRRK2^GS.Scer\UAS and Hsap\LRRK2^KD.Scer\UAS.

(Imai et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26689

y¹ w^67c23; P{UAS-neur.ΔRING::EGFP}8A

Notes on Origin

Discoverer

Comments

Carried in plasmid " UAS::Neur ΔRING" and transfected into S2 cells to study the interaction of the expressed product with the Ser protein.

(Glittenberg et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP}

neur^{ΔRING.UAS.EGFP}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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