nandy5 mutant adults have significantly increased water consumption, compared to controls.
Homozygous flies show disrupted hygrosensing behaviour; a higher fraction of flies choose moist air (100% humidity) over dry air (0% humidity) compared to wild-type controls in a hygrosensory behaviour assay after five minutes.
Mutants show an impaired electrophysiological response to dry air, while leaving moist air sensing unaffected (recordings were taken at the base of the 2nd antennal segment in these experiments).
Homozygotes and nandy5/nan36a transheterozygous adults are viable but show abnormal behaviour: they are sedentary, mildly uncoordinated, more likely than wild-type flies to fall whilst climbing, and slower to right themselves after a fall. Homozygotes show a loss of sound-evoked potentials when the antennal extracellular potentials are recorded. Mutant chordotonal organs appear to have normal ultrastructure.