The vas promoter drives expression of the gus cDNA, fused at the 3' end of the ORF to GFP, followed by the 3'UTR of vas.
gusvas.vas3'UTR.GFP rescues Df(2R)nap14/gusZ409
gusvas.vas3'UTR.GFP rescues gus[+]/gusZ409
Df(2R)nap14/gusZ409 females are rescued to fertility by gusvas.vas3'UTR.T:Avic\GFP. This transgene also rescues eggshell defects and maternal effect cuticle patterning defects due to gusZ409/+.
The product of this construct does not fluoresce because of mutations introduced by PCR during the cloning process.
The product of this construct does not fluoresce because of mutations introduced by PCR during the cloning process.