FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\shotRNAi.UAS
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General Information
Symbol
Dmel\shotRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0150254
Feature type
allele
Associated gene
Dmel\shot
Associated Insertion(s)
Carried in Construct
P{UAS-shot.RNAi}
Also Known As
UAS-shotRNAi
Key Links
Transgenic product class
RNAi_reagent
(Subramanian et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Subramanian et al., 2003)
Mutagen
in vitro construct
(Subramanian et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-shot.RNAi}
Cytology
Description

UAS sequences drive expression of shot sequences.

(Subramanian et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shot
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Wing blisters are seen in 100% of shotdsRNA.Scer\UAS, Scer\GAL4Bx-MS1096 animals. When shotdsRNA.Scer\UAS is driven by Scer\GAL4sr-md710 and Scer\GAL4how-24B, larvae do not grow, remain at first and second instar stages for a few days and eventually die. Tendon cells lose their compact morphology and become very elongated (often three to four times the length of wild-type). However their association with the muscle and with the cuticle is maintained. levels of microtubules (MTs) at muscle-tendon junctions are greatly reduced. Often MTs are significantly elongated in the mutant tendon cell. Also ectopic Actin filaments are formed and detected between the cuticle and the muscle-tendon junction domains. These Actin bundles are independent of MT presence and are detected in cells in which MTs are still observed.

      (Subramanian et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      dsRNA is expressed from the template to study the phenotypic consequences of dsRNA interference (RNAi) of the shot gene.

      (Subramanian et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      shotRNAi.UAS
      shotdsRNA.Scer\UAS
      shotdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (4)