actin filament & nurse cell ring canal
Ring canals in the egg chambers of kelY627A.otu/kelY627A.otu females are narrower than wild-type, but have relatively normal shaped rims. The rate of actin monomer exchange with actin filaments of the nurse cell ring canals is much slower in kelY627A.otu flies than in wild-type.
Rescue of actin cytoskeleton organization and ring canal localization in the oocyte and nurse cells of kelDE1 homozygotes by kelY627A.otu is unaffected by Src64BΔ17/Src64BΔ17. Neither is the abnormal ring canal morphology in these animals.
kelY627A.otu partially rescues kelDE1
Organization of the actin cytoskeleton and ring canal localization in the oocyte and nurse cells of kelDE1 homozygotes is rescued by kelY627A.otu. However, the morphology of these ring canals is not wild-type. Electron microcopy shows that at stage 6, ring canals from wild type and mutant flies have roughly similar morphologies (although mutants have slightly thicker actin bands). However, by stage 10A the ring canals in mutants have distinct differences with wild-type. Light microscopy and actin localisation show that they are significantly narrower than in wild-type, and have a much more concave rim. Electron microscopy shows that the ring canal actin filament numbers are about 50% higher than wild-type, and F-actin remains continuous with no separation into cables as in wild-type; unlike wild-type, the electron-dense material of the outer rim also remains continuous, and folds dramatically into a pointed shape, rather than remaining flat.
