FlyBase Allele Report: Dmel\gig[ΔAkt-P.UAS.Tag:FLAG]

General Information

Symbol

Dmel\gig^{ΔAkt-P.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0150486

Feature type

allele

Associated gene

Dmel\gig

Associated Insertion(s)

Carried in Construct

P{UAS-gig.ΔAkt-P.FLAG}

Key Links

Transgenic product class

missense_variant

(Potter et al., 2002)

polypeptide_post_translational_processing_variant

(Potter et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Potter et al., 2002)

polypeptide_post_translational_processing_variant

(Potter et al., 2002)

Mutagen

in vitro construct

(Potter et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-gig.ΔAkt-P.FLAG}

Cytology

Description

UASt sequences drive expression of gig in which putative Akt1 phosphorylation sites have been mutated (amino acid replacements T437A, S924A, T1054A and T1518A), fused at the N-terminus to the epitope tag Tag:FLAG.

(Potter et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

gig

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^{T231A.S235A.UAS}

(Steinhilb et al., 2007)

modeled by Hsap\MAPT^UAS.cWa

(Steinhilb et al., 2007)

modeled by Hsap\MAPT^{S202A.T205A.UAS}

(Steinhilb et al., 2007)

modeled by Hsap\MAPT^{S396A.S404A.UAS}

(Steinhilb et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Potter et al., 2002)

ommatidium, with Scer\GAL4^ey.PH

(Potter et al., 2002)

wing margin bristle | somatic clone, with Scer\GAL4^Act5C.PI

(Potter et al., 2002)

Detailed Description

Statement

Reference

gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH flies have a 9% decrease in ommatidia numbers compared to wild-type. Wing margin bristles in somatic clones of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^Act5C.PI are smaller than wild-type.

(Potter et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{S396A.S404A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{S202A.T205A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{T231A.S235A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^elav.PLu is a suppressor of increased cell death | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PH, gig^{ΔAkt-P.UAS.Tag:FLAG} has ommatidium phenotype, enhanceable by Tsc1^UAS.Tag:MYC/Scer\GAL4^ey.PH

(Potter et al., 2002)

Scer\GAL4^ey.PH, gig^{ΔAkt-P.UAS.Tag:FLAG} has eye phenotype, enhanceable by Tsc1^UAS.Tag:MYC/Scer\GAL4^ey.PH

(Potter et al., 2002)

Scer\GAL4^Act5C.PI, gig^{ΔAkt-P.UAS.Tag:FLAG} has wing margin bristle | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PI/Tsc1^UAS.Tag:MYC

(Potter et al., 2002)

NOT Enhancer of

Statement

Reference

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Suppressor of

Statement

Reference

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{S396A.S404A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{S202A.T205A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{T231A.S235A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Potter et al., 2002)

gig^{ΔAkt-P.UAS.Tag:FLAG}/Tsc1^UAS.Tag:MYC, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Potter et al., 2002)

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^Act5C.PI is a suppressor of wing margin bristle | somatic clone phenotype of Akt^UAS.Tag:HA, Scer\GAL4^Act5C.PI

(Potter et al., 2002)

gig^{ΔAkt-P.UAS.Tag:FLAG}/Tsc1^UAS.Tag:MYC, Scer\GAL4^Act5C.PI is a suppressor of wing margin bristle | somatic clone phenotype of Akt^UAS.Tag:HA, Scer\GAL4^Act5C.PI

(Potter et al., 2002)

NOT Suppressor of

Statement

Reference

gig^{ΔAkt-P.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Other

Statement

Reference

Scer\GAL4^ey.PH, Tsc1^UAS.Tag:MYC, gig^{ΔAkt-P.UAS.Tag:FLAG} has ommatidium phenotype

(Potter et al., 2002)

Scer\GAL4^ey.PH, Tsc1^UAS.Tag:MYC, gig^{ΔAkt-P.UAS.Tag:FLAG} has eye phenotype

(Potter et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with a 75% decrease in ommatidia numbers compared to wild-type.

gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^GMR.PF; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have a slight (15%) decrease in ommatidial size.

The increase in ommatidial size seen in Akt1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^GMR.PF flies is fully suppressed by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} with or without Tsc1^{Scer\UAS.T:Hsap\MYC}.

The increase in wing margin bristle size seen in Akt1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^Act5C.PI somatic clones is fully suppressed by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} and by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} with Tsc1^{Scer\UAS.T:Hsap\MYC}. In the latter case, the resulting bristles are significantly smaller than wild-type.

The decrease in wing margin bristle size seen in somatic clones of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^Act5C.PI cells, is enhanced by Tsc1^{Scer\UAS.T:Hsap\MYC}.

(Potter et al., 2002)

gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^ey.PH; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have small eyes with a 75% decrease in ommatidia numbers compared to wild-type. gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^GMR.PF; Tsc1^{Scer\UAS.T:Hsap\MYC}/+ flies have a slight (15%) decrease in ommatidial size. The increase in ommatidial size seen in Akt1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^GMR.PF flies is fully suppressed by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} with or without Tsc1^{Scer\UAS.T:Hsap\MYC}. The increase in wing margin bristle size seen in Akt1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^Act5C.PI somatic clones is fully suppressed by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} and by gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} with Tsc1^{Scer\UAS.T:Hsap\MYC}. In the latter case, the resulting bristles are significantly smaller than wild-type. The decrease in wing margin bristle size seen in somaitc clones of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^Act5C.PI cells, is enhanced by Tsc1^{Scer\UAS.T:Hsap\MYC}. Hsap\RPS6KB1^D4.Scer\UAS markedly suppresses the reduction in ommatidial size seen in Tsc1^{Scer\UAS.T:Hsap\MYC}; gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^GMR.PF flies. S6k^Scer\UAS.cWa alone does not.

(Potter et al., 2002)

Xenogenetic Interactions

Statement

Reference

Co-expression of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{S396A.S404A.Scer\UAS}-induced rough eye phenotype.

Co-expression of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{T231A.S235A.Scer\UAS}-induced rough eye phenotype.

Co-expression of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{S202A.T205A.Scer\UAS}-induced rough eye phenotype.

Co-expression of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^Scer\UAS.cWa-induced rough eye phenotype.

(Steinhilb et al., 2007)

Expression of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG} in adult brains expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PLu suppresses increased cell death.

The rough-eye phenotype observed in animals expressing Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} in the eye under the control of Scer\GAL4^GMR.PF is not modified by co-expression of gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}.

(Khurana et al., 2006)

Hsap\RPS6KB1^D4.Scer\UAS markedly suppresses the reduction in ommatidial size seen in Tsc1^{Scer\UAS.T:Hsap\MYC}; gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}; Scer\GAL4^GMR.PF flies. S6k^Scer\UAS.cWa alone does not.

(Potter et al., 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

gig^{ΔAkt-P.Scer\UAS.T:Zzzz\FLAG}

gig^{ΔAkt-P.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections