FlyBase Allele Report: Dmel\hid[UAS.cYa]

General Information

Symbol

Dmel\hid^UAS.cYa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Yoo

FlyBase ID

FBal0150570

Feature type

allele

Associated gene

Dmel\hid

Associated Insertion(s)

Carried in Construct

P{UAS-hid.Y}

Also Known As

UAS-HID, UAS::Hid

Key Links

Transgenic product class

wild_type

(Yoo et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yoo et al., 2002)

Mutagen

in vitro construct

(Olson et al., 2003, Yoo et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-hid.Y}

Cytology

Description

UAS regulatory sequences drive expression of hid.

(Yoo et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

hid

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | conditional, with Scer\GAL4^nan.PK

(Yorozu et al., 2009)

increased cell death, with Scer\GAL4^GMR.AICD.APP

(Gross et al., 2008)

Phenotype Manifest In

chordotonal organ, with Scer\GAL4^nan.PK

(Yorozu et al., 2009)

eye, with Scer\GAL4^GMR.AICD.APP

(Gross et al., 2008)

Detailed Description

Statement

Reference

Ablation of mechanosensory chordotonal neurons via Scer\GAL4^nan.PK-mediated expression of W^Scer\UAS.cYa eliminates the wind-induced suppression of locomotion response.

(Yorozu et al., 2009)

Flies carrying both W^Scer\UAS.cYa and Scer\GAL4^{GMR.ICD.T:Hsap\APP} are viable but exhibit an almost complete absence of eye tissue, due to extensive apoptosis of the cells in the eye.

(Gross et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.AICD.APP, hid^UAS.cYa has increased cell death phenotype, suppressible by X11L^GMR.cGa

(Gross et al., 2008)

Scer\GAL4^GMR.AICD.APP, hid^UAS.cYa has increased cell death phenotype, suppressible by Hsap\APBA2^GMR.cGa

(Gross et al., 2008)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has decreased occurrence of cell division | larval stage phenotype, suppressible by p53^-ns

(Wells et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.AICD.APP, hid^UAS.cYa has increased cell death phenotype, non-suppressible by X11L^ΔPTB.GMR

(Gross et al., 2008)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has increased cell number | larval stage phenotype, non-suppressible by tefu^atm-6

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has increased cell number | larval stage phenotype, non-suppressible by lok^p6

(Wells et al., 2006)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has abnormal size | larval stage phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has increased cell number | larval stage phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has increased cell size | larval stage phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has decreased occurrence of cell division | larval stage phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has abnormal cell death phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, hid^UAS.cYa has visible phenotype

(Huh et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, hid^UAS.cYa has abnormal size | adult stage phenotype

(Huh et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, hid^UAS.cYa has increased occurrence of cell division | larval stage phenotype

(Huh et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.AICD.APP, hid^UAS.cYa has eye phenotype, suppressible by X11L^GMR.cGa

(Gross et al., 2008)

Scer\GAL4^GMR.AICD.APP, hid^UAS.cYa has eye phenotype, suppressible by Hsap\APBA2^GMR.cGa

(Gross et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.AICD.APP, hid^UAS.cYa has eye phenotype, non-suppressible by X11L^ΔPTB.GMR

(Gross et al., 2008)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa, p53^5A-1-4 has wing disc phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa has wing disc phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, hid^UAS.cYa has wing phenotype

(Huh et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, hid^UAS.cYa has wing disc posterior compartment | larval stage phenotype

(Huh et al., 2004)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, hid^UAS.cYa has wing posterior compartment | adult stage phenotype

(Huh et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of X11L^GMR.cGa with W^Scer\UAS.cYa and Scer\GAL4^{GMR.ICD.T:Hsap\APP} almost completely restores eye size to wild-type.

Co-expression of X11L^ΔPTB.GMR with W^Scer\UAS.cYa and Scer\GAL4^{GMR.ICD.T:Hsap\APP} fails to restore eye size to wild-type.

(Gross et al., 2008)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\APBA2^GMR.cGa with W^Scer\UAS.cYa and Scer\GAL4^{GMR.ICD.T:Hsap\APP} almost completely restores eye size to wild-type.

(Gross et al., 2008)

'Undead' cells have been generated in the posterior of the wing disc by simultaneously activating and preventing cell death by coexpression of hid^Scer\UAS.cYa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^hh-Gal4. At 78 hours AEL these wing discs are similar in size, morphology and the pattern of BrdU incorporation to control discs. However, by 96 hours AEL the discs are significantly smaller than controls; the posterior cells and a subset of anterior cells are arrested in G2 and 'undead' posterior cells are nearly 1.73 larger than anterior cells. By the late wandering stage, the 'undead' posterior cells are similar in size to anterior cells, but their cell cycles have shifted to one similar to younger control wing discs, while arrest in anterior cells is permanent. Cell proliferation of the 'undead' cells continues into pupal development and these cells are unable to differentiate and eventually disintegrate.

Neither a tefu^atm-6 or lok^p6 mutant background can alleviate the overgrowth or anterior cell cycle arrest of wing discs with posterior 'undead' Scer\GAL4^hh-Gal4>hid^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells.

The cell cycle arrest and undergrowth phenotype of 96 hour wing discs with posterior 'undead' Scer\GAL4^hh-Gal4>hid^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells is completely alleviated in both p53^5A-1-4 and p53^-ns backgrounds.

'Undead' Scer\GAL4^en-e16E>hid^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells in the posterior of the wing disc show no overgrowth phenotype in a Dronc^I29 background.

(Wells et al., 2006)

Cell proliferation is increased 2- to 4-fold in the posterior compartment of wing discs coexpressing BacA\p35^Scer\UAS.cHa and hid^Scer\UAS.cYa under the control of Scer\GAL4^en-e16E. In the adult, this gives rise to wings with an expansion of the posterior compartment associated with several extra tissue folds. The increased cell proliferation seen in the posterior compartment of wing discs and in adult wings coexpressing BacA\p35^Scer\UAS.cHa and hid^Scer\UAS.cYa under the control of Scer\GAL4^en-e16E is suppressed if they are also coexpressing Dronc^{C318S.Scer\UAS}.

(Huh et al., 2004)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

W^Scer\UAS.cYa

hid^Scer\UAS.cYa

hid^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Yoo

(Yoo et al., 2002)

Secondary FlyBase IDs

References (8)

Report Sections

Open Close