FlyBase Allele Report: Dmel\Spn27A[1]

General Information

Symbol

Dmel\Spn27A¹

Species

D. melanogaster

Name

FlyBase ID

FBal0150599

Feature type

allele

Associated gene

Dmel\Spn27A

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(De Gregorio et al., 2002)

Progenitor genotype

cup^EP2349

(De Gregorio et al., 2002)

Cytology

Description

Mobilization of P{EP}cup^EP2349 caused a 1175bp deletion downstream of the insertion site. This deletion includes the first 275bp of Spn27A.

(De Gregorio et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle | maternal effect (with Df(2L)BSC7)

(Hashimoto et al., 2003)

melanotic mass | adult stage

(Tang et al., 2006)

wing

(De Gregorio et al., 2002)

Detailed Description

Statement

Reference

Mutant animals show spontaneous melanisation.

(Binggeli et al., 2014)

Approximately 6% of homozygotes are viable as adults, and approximately 80% of these escapers show constitutive melanization.

(Tang et al., 2006)

Embryos from Spn27A¹ homozygous mothers do not develop beyond the syncytial blastoderm stage. Embryos from Spn27A¹/Df(2L)BSC7 mothers show a ventralized phenotype and therefore fail to differentiate a cuticle at the end of embryogenesis. This phenotype can be rescued by injection of Spn27A mRNA.

(Hashimoto et al., 2003)

70% of Spn27A¹ mutants die as pupae. Those that survive to adulthood often have wing defects and are female sterile. Ectopic melanisation is seen in mutant adults and larvae in the absence of any injury. Melanization in larvae or adults in response to clean injury, septic injury (with Erwina cartovora) or wasp infection (with L.boulardi), is much more extensive than in similarly treated wild-type animals. In the case of wasp infection or clean injury of larvae, the melanization is usually systemic rather than discrete as in wild-type. 50-70% of mutant larvae pricked with a needle die within 5 hr of injury, compared to 10% of wild-type controls. In most cases, the melanization reaction diffuses throughout the larval body cavity and dead larvae turn completely black. Spn27A¹ homozygotes adults have reduced resistance to natural infection with B.bassiana compared to wild-type (survival after 6 days is around 20% compared to around 90% for controls). However, survival 6 days after infection by pricking with cultures of E.coli, M.luteus, or A.fumigata is similar to that after clean injury of mutants.

(De Gregorio et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Spn27A¹ has melanotic mass phenotype phenotype, enhanceable by PPO2^Δ

(Binggeli et al., 2014)

Spn27A¹ has partially lethal phenotype, enhanceable by PPO2^Δ

(Binggeli et al., 2014)

Suppressed by

Statement

Reference

Spn27A¹ has melanotic mass phenotype phenotype, suppressible by PPO1^Δ/PPO2^Δ

(Binggeli et al., 2014)

Spn27A¹ has partially lethal phenotype, suppressible by PPO1^Δ/PPO2^Δ

(Binggeli et al., 2014)

Spn27A¹ has melanotic mass phenotype | adult stage phenotype, suppressible by Df(3R)ME15/+

(Tang et al., 2006)

Spn27A¹ has melanotic mass phenotype | adult stage phenotype, suppressible by Df(3R)dsx11/+

(Tang et al., 2006)

Spn27A¹ has partially lethal - majority die | recessive | pupal stage phenotype, suppressible by PPO1^Bc

(De Gregorio et al., 2002)

NOT suppressed by

Statement

Reference

Spn27A¹ has female sterile phenotype, non-suppressible by PPO1^Bc

(De Gregorio et al., 2002)

NOT Suppressor of

Statement

Reference

Spn27A¹ is a non-suppressor of abnormal immune response | adult stage phenotype of PPO1^Bc

(De Gregorio et al., 2002)

Other

Statement

Reference

PPO1^Δ, Spn27A¹ has melanotic mass phenotype phenotype

(Binggeli et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Spn27A¹ has melanotic mass | adult stage phenotype, suppressible by Df(3R)ME15/+

(Tang et al., 2006)

Spn27A¹ has melanotic mass | adult stage phenotype, suppressible by Df(3R)dsx11/+

(Tang et al., 2006)

Spn27A¹/Df(2L)BSC7 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by spz^D1-RPQ/Df(3R)Tl-D

(Hashimoto et al., 2003)

Spn27A¹/Df(2L)BSC7 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by ea⁴/Df(3R)ea-5022rx1

(Hashimoto et al., 2003)

Spn27A¹ has wing phenotype, suppressible by PPO1^Bc

(De Gregorio et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The spontaneous melanisation seen in Spn27A¹ mutant animals is suppressed if they are also mutant for both PPO1^Δ and PPO2^Δ.

50% of Spn27A¹ PPO1^Δ double mutant larvae and pupae show a characteristic pattern of melanisation spots under the epidermis.

The spontaneous melanisation seen in Spn27A¹ mutant animals is stronger if they are also mutant for PPO2^Δ; the double mutant pupae turn black and adults have large spots and wing defects.

(Binggeli et al., 2014)

The constitutive melanization phenotype of Spn27A¹ homozygous escapers is suppressed more than 5-fold if they are also heterozygous for Df(3R)ME15 or Df(3R)dsx11.

(Tang et al., 2006)

The ventralized phenotype that causes the failure of cuticle differentiation in embryos from Spn27A¹/Df(2L)BSC7 mothers is abolished when mothers also have the mutant genotype ea⁴/Df(3R)ea-5022rx1 or spz^D1-RPQ/Df(3R)Tl-D. Such embryos have a dorsalized phenotype that is indistinguishable from those produced by single mutant ea⁴/Df(3R)ea-5022rx1 or spz^D1-RPQ/Df(3R)Tl-D mothers.

(Hashimoto et al., 2003)

Pupal lethality, wing phenotypes and ectopic melanisation in larvae after clean injury in Spn27A¹ mutants are all suppressed by homozygosity for Bc¹. The female sterility phenotype of Spn27A¹ mutants is not suppressed.

The melanization response in Bc¹ homozygotes is unaffected by Spn27A¹, as is the reduced resistance to natural infection with B.bassiana.

Pupal lethality, wing phenotypes and ectopic melanisation in larvae after clean injury in Spn27A¹ mutants are all suppressed by homozygosity for Bc^unspecified. The female sterility phenotype of Spn27A¹ mutants is not suppressed.

(De Gregorio et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Recombinant Spn27A protein purified from S2 cells transfected with Spn27A^{MtnA.T:SV5\V5,T:Zzzz\His6}, rescues ectopic melanisation due to clean injury of Spn27A¹ homozygous adults, when injected into the injury site.

(De Gregorio et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

55720

w^*; Spn27A¹/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

Comments

Spn27A¹ complements cup^unspecified : Unlike Spn27A¹ or cup^unspecified homozygotes, Spn27A¹/cup^unspecified females are not sterile.

(De Gregorio et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Spn27A1

(Chen and He, 2022)

Spn27A¹

(Binggeli et al., 2014, Nappi et al., 2005)

spn27A¹

(Tang et al., 2006, Hashimoto et al., 2003, De Gregorio et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (8)

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