FlyBase Allele Report: Dmel\Moe[TA.UAS.EGFP]

General Information

Symbol

Dmel\Moe^TA.UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0150742

Feature type

allele

Associated gene

Dmel\Moe

Associated Insertion(s)

Carried in Construct

P{UAS-Moe.TA::GFP}

Key Links

Transgenic product class

missense_variant

(Polesello et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Polesello et al., 2002)

Mutagen

in vitro construct

(Polesello et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Moe.TA::GFP}

Cytology

Description

Expression of mutated full length Moe (carries the amino acid replacement T559A), fused at the C-terminal to EGFP is under the control of UAS sequences.

(Polesello et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Moe

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Moe^PL106, Scer\GAL4^αTub84B.PL

(Roch et al., 2010)

Phenotype Manifest In

cyst progenitor cell, with Scer\GAL4^C587

(Cheng et al., 2011)

cyst progenitor cell | somatic clone, with Scer\GAL4^Act5C.PI

(Cheng et al., 2011)

eye, with Moe^PL106, Scer\GAL4^αTub84B.PL

(Roch et al., 2010)

nurse cell & actin filament, with Scer\GAL4^Moe-PG26

(Polesello et al., 2002)

nurse cell & actin filament, with Scer\GAL4^{VP16.nanos.UTR}

(Polesello et al., 2002)

oocyte & actin filament | ectopic, with Scer\GAL4^{VP16.nanos.UTR}

(Polesello et al., 2002)

spindle, with Scer\GAL4^C587

(Cheng et al., 2011)

Detailed Description

Statement

Reference

Only 18% of testis somatic cyst stem cells show repositioning of the mitotic spindle during anaphase in males expressing Moe^{TA.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^C587 compared to 76% of cases in heterozygous controls.

Testis somatic cyst cell clones expressing Moe^{TA.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PI only divide asymmetrically in 58% of cases (in contrast to 96% of cases in control somatic cyst cell clones), in the remainder of cases, both daughters attach to the hub.

(Cheng et al., 2011)

While expression of Moe^{TA.Scer\UAS.T:Avic\GFP-EGFP} using Scer\GAL4^αTub84B.PL fully rescues the wing phenotypes of Moe^PL106, it only partially rescues the viability, and fails to rescue the eye phenotypes.

(Roch et al., 2010)

The oocytes of Moe^{TA.Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} at stage 8-9 have ectopic clumps of actin in the cytoplasm. In the nurse cells of Moe^{TA.Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} or Moe^{TA.Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^Moe-PG26 females the actin filaments are dramatically rearranged from a cortical mesh into elaborate flower-like structures.

(Polesello et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Moe^TA.UAS.EGFP/Scer\GAL4^αTub84B.PL partially rescues Moe^PL106

(Roch et al., 2010)

Fails to rescue

Moe^TA.UAS.EGFP/Scer\GAL4^αTub84B.PL fails to rescue Moe^PL54

(Roch et al., 2010)

Comments

(Roch et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Moe^{TA.Scer\UAS.T:Avic\GFP-EGFP}

Moe^TA.UAS.EGFP

Name Synonyms

non-phosphorylated Dmoesin-GFP

(Miller, 2003)

Secondary FlyBase IDs

References (7)

Report Sections

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