FlyBase Allele Report: Dmel\Mhc[2B]

General Information

Symbol

Dmel\Mhc^2B

Species

D. melanogaster

Name

FlyBase ID

FBal0150764

Feature type

allele

Associated gene

Dmel\Mhc

Associated Insertion(s)

Carried in Construct

Also Known As

Mhc^P401S

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Nongthomba et al., 2003)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T. Amino acid replacement: P401S.

(Nongthomba et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:16,774,654..16,774,654 [+]

Nucleotide change:

C16774654T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

P401S

(Nongthomba et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | dominant

(Nongthomba et al., 2003)

flightless | dominant

(Nongthomba et al., 2003)

lethal (with Df(2L)H20)

(Nongthomba et al., 2003)

lethal (with Mhc¹)

(Nongthomba et al., 2003)

lethal | recessive

(Nongthomba et al., 2003)

visible (with Mhc¹³)

(Nongthomba et al., 2003)

Phenotype Manifest In

indirect flight muscle cell (with Mhc¹³)

(Nongthomba et al., 2003)

wing (with Mhc¹³)

(Nongthomba et al., 2003)

Detailed Description

Statement

Reference

Heterozygous flies show age-related myopathy in their walking behaviour. 4% of Mhc^2B/Mhc¹³ adults have an upheld wing phenotype, 38% have their wings held down and 58% hold their wings in the normal position. 0% of the indirect flight muscle fibres of mutant adults are hypercontracted, 35% show a partial hypercontraction phenotype and 65% are normal.

(Nongthomba et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Mhc^2B is a suppressor | partially of decreased size phenotype of Scer\GAL4^Mhc.PU, bru1^UAS.cOa

(Nikonova et al., 2022)

Mhc[+]/Mhc^2B is a suppressor of visible phenotype of fln⁰

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of visible phenotype of up¹⁰¹

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of abnormal locomotor behavior phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of visible phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

NOT Suppressor of

Statement

Reference

Mhc¹²/Mhc^2B is a non-suppressor of visible phenotype of wupA^hdp-3

(Nongthomba et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

Mhc^2B is a suppressor of indirect flight muscle cell phenotype of Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Mhc^2B is a suppressor of muscle cell | decreased number phenotype of Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Mhc^2B is a suppressor of dorsal longitudinal indirect flight muscle cell phenotype of Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Mhc^2B is a suppressor | partially of muscle cell | absent phenotype of Scer\GAL4^Mhc.PU, bru1^UAS.cOa

(Nikonova et al., 2022)

Mhc^2B is a suppressor | partially of myofibril phenotype of Scer\GAL4^Mhc.PU, bru1^UAS.cOa

(Nikonova et al., 2022)

Mhc^2B is a suppressor | partially of sarcomere phenotype of Scer\GAL4^Mhc.PU, bru1^UAS.cOa

(Nikonova et al., 2022)

Mhc^2B is a suppressor of sarcomere phenotype of Rbfox1^RNAi.UAS, Scer\GAL4^Mef2.PR

(Nikonova et al., 2022)

Mhc^2B/Mhc^2B is a suppressor of dorsal longitudinal indirect flight muscle cell phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

Mhc^2B/Mhc^2B is a suppressor of myofibril | adult stage phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

Mhc^2B/Mhc^2B is a suppressor of sarcomere | adult stage phenotype of Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC

(Katti et al., 2017)

Mhc[+]/Mhc^2B is a suppressor of dorsal longitudinal indirect flight muscle cell | heat sensitive phenotype of wupA^fliH

(Firdaus et al., 2015)

Mhc[+]/Mhc^2B is a suppressor of indirect flight muscle cell phenotype of fln⁰

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of wing phenotype of fln⁰

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of indirect flight muscle cell phenotype of up¹⁰¹

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of wing phenotype of up¹⁰¹

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of indirect flight muscle cell phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

Mhc[+]/Mhc^2B is a suppressor of wing phenotype of wupA^hdp-2

(Nongthomba et al., 2003)

NOT Suppressor of

Statement

Reference

Mhc¹²/Mhc^2B is a non-suppressor of wing phenotype of wupA^hdp-3

(Nongthomba et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The severe dorsal longitudinal muscle (DLM) structural defects observed in adult flies expressing mir-9a^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^Hk-UH3 are suppressed by combination with Mhc^2B.

(Katti et al., 2017)

In the thorax of wupA^hdp-3/Y; Mhc⁷/Mhc^2B flies there is an absence of myofibrils and no clear areas of interdigitated thick-thin filaments. There are visible "tiger-tail" structures formed by condensed serially repeated Z-discs connected by short stretches of thin filaments.

(Nongthomba et al., 2004)

wupA^hdp-2/Y ; Mhc^2B/+ flies show a complete recovery of wild-type myofibrillar structure in the indirect flight muscles, although the sarcomere length remains slightly, but significantly shorter than that of wild type. The defects seen in fln⁰ flies are partially suppressed by Mhc^2B/+; 22% of the double mutant adults have an upheld wing phenotype and 78% hold their wings in the normal position. The indirect flight muscle fibres are normal in these flies. The indirect flight muscles of wupA^hdp-3/Y ; Mhc¹²/Mhc^2B flies show a partial hypercontraction phenotype.

(Nongthomba et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Mhc¹

(Nongthomba et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a dominant suppressor of the wupA^hdp-2 wings-up phenotype. Mutations "Su(2)A", "Su(2)B" and "Su(2)C" contain the same lesion and are assumed to have arisen from a single mutational event.

(Nongthomba et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Mhc^2B

(Firdaus et al., 2015)

Mhc^P401S

(Nikonova et al., 2022, Katti et al., 2017, Firdaus et al., 2015, Nongthomba et al., 2004)

Su(2)A

(Nongthomba et al., 2003)

Su(2)B

(Nongthomba et al., 2003)

Su(2)C

(Nongthomba et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close