FlyBase Allele Report: Dmel\mir-9a[UAS.pLUC]

General Information

Symbol

Dmel\mir-9a^UAS.pLUC

Species

D. melanogaster

Name

FlyBase ID

FBal0277364

Feature type

allele

Associated gene

Dmel\mir-9a

Associated Insertion(s)

Carried in Construct

P{UAS-LUC-mir-9a.T}

Also Known As

UAS-miR-9a

Key Links

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

(Bejarano et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-LUC-mir-9a.T}

Cytology

Description

UAS regulatory sequences drive expression of Ppyr\LUC followed by a genomic fragment containing the mir-9a pre-miRNA hairpin.

(Bejarano et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-9a

Tagged with

pLUC

bioluminescence reporter

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

visible, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

visible | adult stage, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

myofibril | adult stage, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

sarcomere | adult stage, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

wing, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

wing, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

wing margin, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

Detailed Description

Statement

Reference

Adult flies expressing mir-9a^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^Hk-UH3 show wing posture defects and are completely flightless. Their dorsal longitudinal muscles are severely disrupted with broken muscle fibers that appear hypercontracted and whole fascicles missing, the sarcomere is disorganized and no clear Z-discs are formed.

(Katti et al., 2017)

Expression of mir-9a^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^sd-SG29.1 results in curved, spoon-shaped wings which are slightly folded. The distribution of hairs on the wing margin is not homogeneous.

(Bejarano et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has flightless phenotype, suppressible | partially by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has flightless phenotype, suppressible | partially by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has sarcomere | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by Mhc^2B/Mhc^2B

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has myofibril | adult stage phenotype, suppressible by Mhc^2B/Mhc^2B

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has sarcomere | adult stage phenotype, suppressible by Mhc^2B/Mhc^2B

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has myofibril | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has sarcomere | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10a}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has dorsal longitudinal indirect flight muscle cell phenotype, suppressible by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Scer\GAL4^Hk-UH3, mir-9a^UAS.pLUC has myofibril | adult stage phenotype, suppressible by up^{Δmir-9a.UAS.10b}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The severe dorsal longitudinal muscle (DLM) structural defects observed in adult flies expressing mir-9a^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^Hk-UH3 are suppressed by combination with Mhc^2B. Similarly, the disrupted DLM morphology, along with the sarcomere disorganization and to a large extent also the flightlessness of mir-9a^{Scer\UAS.T:Ppyr\LUC}-expressing flies is rescued by co-expression of either up^{Δmir-9a.Scer\UAS.10a} or up^{Δmir-9a.Scer\UAS.10b}.

(Katti et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mir-9a^UAS.pLUC is rescued by mir-9a^{sponge.UAS.mCherry}/Scer\GAL4^Hk-UH3

(Katti et al., 2017)

Comments

The inability to fly along with severe structural defects in their indirect flight muscles (broken muscle fibers, disorganized sarcomere) characteristic for flies expressing mir-9a^{Scer\UAS.T:Ppyr\LUC} under the control of Scer\GAL4^Hk-UH3 is rescued by co-expression of mir-9a^{sponge.Scer\UAS.T:Disc\RFP-mCherry}.

(Katti et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

41138

w¹¹¹⁸; P{UAS-LUC-mir-9a.T}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-9a^{Scer\UAS.T:Ppyr\LUC}

mir-9a^UAS.pLUC

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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