An Hsp70 promoter drives expression of the complete rl coding sequence, followed by amino acids 2-147 of Scer\GAL4 (includes the DNA-binding domain), followed by residues 413-490 of Hsim\VP16 (activation domain), followed by a Tag:HSV-1-gD epitope tag.
rlMG.hs.Tag:HSV-1-gD partially rescues rl1
Expression of rlhs.T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD at 29oC strongly suppresses the eye defects seen in rl1 animals.