An Hsp70 promoter drives expression of the complete rl coding sequence, tagged at the C-terminal end with Tag:HSV-1-gD.
Ectopic expression of rlhs.T:Hsim\gD for 1 hour at various times during development has no effect on the pattern of veins in the adult wing. Similarly, when rlhs.T:Hsim\gD flies are raised continuously at 29oC, no effects are seen in wing vein patterning. Ectopic expression of rlhs.T:Hsim\gD between late L3 and mid pupation (96-168 hours after egg deposition) does not produce blisters or any increase in cell division in the wing.
rlhs.Tag:HSV-1-gD partially rescues rl1