FlyBase Allele Report: Dmel\polo[T182D.UAS]

General Information

Symbol

Dmel\polo^T182D.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0154736

Feature type

allele

Associated gene

Dmel\polo

Associated Insertion(s)

Carried in Construct

P{UAS-polo.T182D}

Also Known As

UAS-polo^T182D

Key Links

Transgenic product class

missense_variant

(Parks, 2004.5.12)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Parks, 2004.5.12)

Mutagen

in vitro construct

(Parks, 2004.5.12)

Progenitor genotype

Carried in construct

P{UAS-polo.T182D}

Cytology

Description

UAS regulatory sequences drive expression of a mutated form of polo, carrying the amino acid replacement T182D, which is predicted to be constitutively active. The mutation is equivalent to a T210D change in the human ortholog.

(Parks, 2004.5.12)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

polo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

lethal - all die during embryonic stage, with Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

some die during embryonic stage, with Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Phenotype Manifest In

type II neuroblast | somatic clone | increased number, with Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Detailed Description

Statement

Reference

Expression of the constitutively active polo^{T18D.Scer\UAS} in the brain under the control of Scer\GAL4^Dll-md23 causes embryonic lethality.

Expression of the constitutively active polo^{T18D.Scer\UAS} in neuroblast clones induces ectopic type II neuroblasts.

(Ouyang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Dll-md23, polo^T182D.UAS has abnormal neuroanatomy phenotype, suppressible by numb^TS4A.UAS/Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Dll-md23, polo^T182D.UAS has type II neuroblast | increased number | somatic clone phenotype, suppressible by numb^TS4A.UAS/Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Suppressor of

Statement

Reference

polo^T182D.UAS/Scer\GAL4^Mef2.PR is a suppressor of dorsal transverse muscle cell | embryonic stage phenotype of bsd¹

(Yang et al., 2022)

polo^T182D.UAS/Scer\GAL4^Mef2.PR is a suppressor of muscle cell of lateral oblique muscle 1 | embryonic stage phenotype of bsd¹

(Yang et al., 2022)

polo^T182D.UAS/Scer\GAL4^Mef2.PR is a suppressor of muscle cell of ventral longitudinal muscle 1 | embryonic stage phenotype of bsd¹

(Yang et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of numb^{TS4A.Scer\UAS} suppresses the induction of ectopic neuroblast in type II neuroblast clones expressing polo^{T18D.Scer\UAS}.

(Ouyang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8434

y¹ w¹¹¹⁸; P{UAS-polo.T182D}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

polo^{T182D.Scer\UAS}

polo^T182D.UAS

polo^{T18D.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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