FlyBase Allele Report: Dmel\pie[E1-16]

General Information

Symbol

Dmel\pie^E1-16

Species

D. melanogaster

Name

FlyBase ID

FBal0154763

Feature type

allele

Associated gene

Dmel\pie

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Shi et al., 2003)

Progenitor genotype

Cytology

Description

A 13bp deletion (nucleotides 644-656 of cDNA) leads to a frameshift at Asp203 adding 16 novel amino acids followed by a stop codon.

(Shi et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:10,421,963..10,421,975 [-]

Comment:

A 13bp deletion (nucleotides 644-656 of cDNA) leads to a frameshift at Asp203 adding 16 novel amino acids followed by a stop codon. The location of the deletion was mapped relative to the cDNA from GB:AF247501.

(Shi et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with pie^EB3)

(Shi et al., 2003)

increased cell death | somatic clone

(Xing et al., 2015)

lethal (with Df(2L)J77)

(Shi et al., 2003)

lethal (with pie^E1-2)

(Shi et al., 2003)

lethal (with pie^G2-4)

(Shi et al., 2003)

lethal (with pie^H113)

(Shi et al., 2003)

lethal | recessive

(Shi et al., 2003)

sterile (with pie⁵⁴)

(Shi et al., 2003)

sterile (with pie^EB3)

(Shi et al., 2003)

sterile (with pie^QM47)

(Shi et al., 2003)

visible (with pie^EB3)

(Xing et al., 2015)

Phenotype Manifest In

cone cell | somatic clone

(Shi et al., 2003)

eye (with pie⁵⁴)

(Shi et al., 2003)

eye (with pie^EB3)

(Xing et al., 2015, Shi et al., 2003)

eye (with pie^QM47)

(Shi et al., 2003)

eye | somatic clone

(Shi et al., 2003)

eye disc | somatic clone

(Xing et al., 2015)

female germline stem cell | germline clone

(Xing et al., 2015)

male germline stem cell | germline clone

(Xing et al., 2015)

ommatidium (with pie⁵⁴)

(Shi et al., 2003)

ommatidium (with pie^EB3)

(Shi et al., 2003)

ommatidium (with pie^QM47)

(Shi et al., 2003)

ommatidium | somatic clone

(Shi et al., 2003)

photoreceptor neuron | somatic clone

(Shi et al., 2003)

Detailed Description

Statement

Reference

pie^E1-16/pie^EB3 mutant adult survivors exhibit a marked rough eye phenotype.

pie^E1-16 mutant eye disc clones exhibit increased apoptosis compared with wild type controls, and the resulting eyes contain very few mutant clones.

pie^E1-16 mutant male germline stem cell clones are seen at two days after induction but rapidly lost by 5-7 days.

The division rate of pie^E1-16 mutant female germline stem cell clones is reduced compared to controls, and the cell cycle is disrupted.

pie^E1-16 intestinal stem cell clones in the midgut generate significantly fewer cells compared with wild-type clones.

(Xing et al., 2015)

Somatic clones of pie^E1-16 cells in the eye disc have many apoptotic cells (evenly distributed throughout the clone) and small ommatidia with a variable shortfall in numbers of photoreceptors and cone cells. In adult eyes these clones are rough.

(Shi et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

pie^E1-16 has increased cell death | somatic clone phenotype, suppressible by foxo²⁵/foxo[+]

(Xing et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

pie^E1-16 has male germline stem cell | germline clone phenotype, suppressible by foxo²⁵/foxo[+]

(Xing et al., 2015)

pie^E1-16 has eye disc | somatic clone phenotype, suppressible by foxo²⁵/foxo[+]

(Xing et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of foxo²⁵ significantly suppresses the male germline stem cell (GSC) loss and reduced GSC division rate seen in pie^E1-16 mutant clones.

One copy of foxo²⁵ significantly suppresses the increased apoptosis seen in pie^E1-16 mutant eye disc clones, and clones are seen in adult eyes.

(Xing et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Clegg

(Shi et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pie^E1-16

(Xing et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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