FlyBase Allele Report: Dmel\kek5[UAS.GFP]

General Information

Symbol

Dmel\kek5^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0155156

Feature type

allele

Associated gene

Dmel\kek5

Associated Insertion(s)

Carried in Construct

P{UAS-kek5.GFP}

Key Links

Transgenic product class

wild_type

(Alvarado et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Alvarado et al., 2004)

Mutagen

in vitro construct

(Alvarado et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-kek5.GFP}

Cytology

Description

UAS regulatory sequences drive expression of kek5 tagged at the C-terminal end with GFP.

(Alvarado et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

kek5

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

abnormal size, with Scer\GAL4^en-e16E

(Evans et al., 2009)

abnormal size, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

visible, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

visible, with Scer\GAL4^en-e16E

(Evans et al., 2009)

visible, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

anterior crossvein, with Scer\GAL4^en-e16E

(Evans et al., 2009)

anterior crossvein, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

posterior crossvein, with Scer\GAL4^en-e16E

(Evans et al., 2009)

scutellar bristle | increased number, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

wing, with Scer\GAL4^en-e16E

(Evans et al., 2009)

wing, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

wing blade, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

wing blade, with Scer\GAL4^en-e16E

(Evans et al., 2009)

wing blade, with Scer\GAL4^ptc-559.1

(Evans et al., 2009)

Detailed Description

Statement

Reference

Expression of kek5^{Scer\UAS.T:Avic\GFP} throughout the wing driven by either Scer\GAL4^Bx-MS1096, Scer\GAL4^en-e16E or Scer\GAL4^ptc-559.1 prevents formation of the anterior crossvein (ACV) and results in decreased wing size. These phenotypes are highly penetrant. Scer\GAL4^en-e16E driven expression of kek5^{Scer\UAS.T:Avic\GFP} also causes the loss of the posterior crossvein in addition to the ACV.

Supernumerary scutellar bristles are induced in response to expression of kek5^{Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^ptc-559.1.

(Evans et al., 2009)

Expression of kek5^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^CY2 in follicle cells has no effect on dorsoventral patterning of the chorion.

(Alvarado et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/kek5^UAS.GFP is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, gbb^UAS.cKa

(Evans et al., 2009)

Scer\GAL4^ptc-559.1/kek5^UAS.GFP is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, sog^UAS.cYa

(Evans et al., 2009)

Other

Statement

Reference

kek5^UAS.GFP, sax^{Q263D.UAS.Tag:HA} has visible | recessive phenotype

(Evans et al., 2009)

kek5^UAS.GFP, tkv^{Q199D.UAS.Tag:HA} has visible | recessive phenotype

(Evans et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

kek5^UAS.GFP is an enhancer of wing cell phenotype of tkv^{Q199D.UAS.Tag:HA}

(Evans et al., 2009)

kek5^UAS.GFP is an enhancer of wing vein phenotype of tkv^{Q199D.UAS.Tag:HA}

(Evans et al., 2009)

kek5^UAS.GFP is an enhancer of wing cell phenotype of sax^{Q263D.UAS.Tag:HA}

(Evans et al., 2009)

kek5^UAS.GFP is an enhancer of wing vein phenotype of sax^{Q263D.UAS.Tag:HA}

(Evans et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/kek5^UAS.GFP is a suppressor of anterior crossvein phenotype of Scer\GAL4^ptc-559.1, gbb^UAS.cKa

(Evans et al., 2009)

Scer\GAL4^ptc-559.1/kek5^UAS.GFP is a suppressor of crossvein phenotype of Scer\GAL4^ptc-559.1, sog^UAS.cYa

(Evans et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The ectopic crossvein phenotype in wings expressing sog^Scer\UAS.cYa driven by Scer\GAL4^ptc-559.1 can be suppressed by co-expressing kek5^{Scer\UAS.T:Avic\GFP}.

The ectopic vein phenotype in the area where the anterior crossvein normally forms resulting from the Scer\GAL4^ptc-559.1 driven expression of gbb^Scer\UAS.cKa can be suppressed by co-expressing kek5^{Scer\UAS.T:Avic\GFP} in the wing.

The wing patterning phenotypes resulting from the expression of tkv^{Q199D.Scer\UAS.T:Ivir\HA1} with Scer\GAL4^ptc-559.1 are enhanced by the co-expression of kek5^{Scer\UAS.T:Avic\GFP}, and there is an additional decrease in wing size.

The wing patterning phenotypes resulting from the expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} with Scer\GAL4^ptc-559.1 are enhanced by the co-expression of kek5^{Scer\UAS.T:Avic\GFP}, and there is an additional decrease in wing size.

(Evans et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S3 cells for co-immunoprecipitation experiments.

(Alvarado et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

kek5^{Scer\UAS.T:Avic\GFP}

kek5^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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