FlyBase Allele Report: Dmel\sax[Q263D.UAS.Tag:HA]

General Information

Symbol

Dmel\sax^{Q263D.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0092532

Feature type

allele

Associated gene

Dmel\sax

Associated Insertion(s)

Carried in Construct

P{UAS-sax.Q263D.T:HA1}

Also Known As

UAS-sax^A, UAS-saxA, UAS-sax^act, UAS-sax^Q263D

Key Links

Transgenic product class

missense_variant

(Haerry et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Haerry et al., 1998)

Mutagen

in vitro construct

(Haerry et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-sax.Q263D.T:HA1}

Cytology

Description

An activated form of sax, with the amino acid replacement Q263D, and tagged with Tag:HA is expressed under the control of UAS regulatory sequences.

(Haerry et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

sax

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^A9

(Haerry, 2010, Haerry et al., 1998)

visible | recessive

(Evans et al., 2009)

Phenotype Manifest In

posterior crossvein, with Scer\GAL4^A9

(Fischer et al., 2012)

wing, with Scer\GAL4^A9

(Haerry et al., 1998)

wing cell

(Evans et al., 2009)

wing vein

(Evans et al., 2009)

wing vein, with Scer\GAL4^A9

(Haerry, 2010, Haerry et al., 1998)

wing vein | ectopic, with Scer\GAL4^A9

(Fischer et al., 2012, Haerry et al., 1998)

Detailed Description

Statement

Reference

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in Pdf-positive neurons, under the control of Scer\GAL4^P2.4.Pdf does not affect circadian rhythm.

(Beckwith et al., 2013)

Overexpression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} with Scer\GAL4^A9 leads to extra vein material between the region of L3 and L5 with slight wing blistering, also affecting the posterior crossvein pattern.

(Fischer et al., 2012)

Pan-neuronal expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} does not generate appreciable motor axon phenotypes.

(Miller et al., 2011)

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in the wing under the control of Scer\GAL4^A9 results in mild wing-vein patterning defects.

(Haerry, 2010)

Scer\GAL4^GMR.PF-driven overexpression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} has no effect on eye development.

(Saja et al., 2010)

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in the wing with Scer\GAL4^ptc-559.1 induces patterning abnormalities including extensive ectopic vein, fusion of longitudinal veins, and loss of intervein regions.

(Evans et al., 2009)

Expression of tkv^{Q199D.UAS.Tag:HA}, under the control of either Scer\GAL4^ChAT.7.4, or Scer\GAL4^eve.RN2 has no effect on the synaptic current amplitude in aCC/RP2 neurons compared to controls.

(Baines, 2004)

Flies expressing sax^{Q263D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 have ectopic and thickened wing veins with a small amount of wing blistering in the region of the posterior crossvein.

(Haerry et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA} has visible phenotype, non-enhanceable by Mad^SDVD.UAS, Scer\GAL4^A9

(Haerry, 2010)

Enhancer of

Statement

Reference

Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA} is an enhancer of visible phenotype of Scer\GAL4^A9, tkv^{1-A-T318D.UAS}

(Haerry, 2010)

Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA} is an enhancer of visible phenotype of Scer\GAL4^A9, tkv^{1-A-T485D.UAS}

(Haerry, 2010)

Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA} is an enhancer of visible phenotype of Scer\GAL4^A9, tkv^{1AΔGS.UAS.Tag:HA}

(Haerry, 2010)

sax^{Q263D.UAS.Tag:HA}, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA}, Scer\GAL4^how-24B is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^elav-C155, Scer\GAL4^how-24B, frac^UAS.cMa

(Miller et al., 2011)

sax^{Q263D.UAS.Tag:HA}/Scer\GAL4^how-24B is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^how-24B, frac^UAS.cMa

(Miller et al., 2011)

Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA} is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^elav-C155, frac^UAS.cMa

(Miller et al., 2011)

Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA} is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

sax^{Q263D.UAS.Tag:HA}/Scer\GAL4^RapGAP1-OK6 is a suppressor of abnormal neurophysiology phenotype of Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^G14/sax^{Q263D.UAS.Tag:HA} is a non-suppressor of abnormal neurophysiology phenotype of Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

Other

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, sax^{Q263D.UAS.Tag:HA}, tkv^{Q199D.UAS.Tag:HA} has increased cell number | adult stage phenotype

(Smendziuk et al., 2015)

Scer\GAL4^P2.4.Pdf, sax^{Q263D.UAS.Tag:HA}, tkv^{Q253D.UAS.cNb} has abnormal circadian rhythm phenotype

(Beckwith et al., 2013)

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA}, tkv^{1-A-T318D-T485D.UAS} has visible phenotype

(Haerry, 2010)

kek5^UAS.GFP, sax^{Q263D.UAS.Tag:HA} has visible | recessive phenotype

(Evans et al., 2009)

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA}, tkv^{DE-Q199D.tld.Tag:HA} has visible phenotype

(Haerry et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

sax^{Q263D.UAS.Tag:HA} has wing cell phenotype, enhanceable by kek5^UAS.GFP

(Evans et al., 2009)

sax^{Q263D.UAS.Tag:HA} has wing vein phenotype, enhanceable by kek5^UAS.GFP

(Evans et al., 2009)

NOT Enhanced by

Statement

Reference

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA} has wing vein phenotype, non-enhanceable by Mad^SDVD.UAS, Scer\GAL4^A9

(Haerry, 2010)

Suppressed by

Statement

Reference

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA} has wing vein | ectopic phenotype, suppressible | partially by Scer\GAL4^A9/fuss^UAS.C

(Fischer et al., 2012)

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA} has posterior crossvein phenotype, suppressible | partially by Scer\GAL4^A9/fuss^UAS.C

(Fischer et al., 2012)

Enhancer of

Statement

Reference

Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA} is an enhancer of wing phenotype of Scer\GAL4^A9, tkv^{1-A-T318D.UAS}

(Haerry, 2010)

Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA} is an enhancer of wing vein phenotype of Scer\GAL4^A9, tkv^{1-A-T485D.UAS}

(Haerry, 2010)

Scer\GAL4^A9/sax^{Q263D.UAS.Tag:HA} is an enhancer of wing vein phenotype of Scer\GAL4^A9, tkv^{1AΔGS.UAS.Tag:HA}

(Haerry, 2010)

sax^{Q263D.UAS.Tag:HA}, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA} is a suppressor of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA} is a suppressor of larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA}, Scer\GAL4^how-24B is a suppressor | partially of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Scer\GAL4^elav-C155, Scer\GAL4^how-24B, frac^UAS.cMa

(Miller et al., 2011)

sax^{Q263D.UAS.Tag:HA}/Scer\GAL4^how-24B is a suppressor | partially of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Scer\GAL4^how-24B, frac^UAS.cMa

(Miller et al., 2011)

Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA} is a suppressor | partially of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Scer\GAL4^elav-C155, frac^UAS.cMa

(Miller et al., 2011)

sax^{Q263D.UAS.Tag:HA}/Scer\GAL4^RapGAP1-OK6 is a suppressor of bouton phenotype of Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^G14/sax^{Q263D.UAS.Tag:HA} is a non-suppressor of bouton phenotype of Impβ11⁷⁰

(Higashi-Kovtun et al., 2010)

Other

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, sax^{Q263D.UAS.Tag:HA}, tkv^{Q199D.UAS.Tag:HA} has somatic cell of testis phenotype

(Smendziuk et al., 2015)

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA}, tkv^{DE-Q199D.tld.Tag:HA} has wing phenotype

(Haerry et al., 1998)

Scer\GAL4^A9, sax^{Q263D.UAS.Tag:HA}, tkv^{DE-Q199D.tld.Tag:HA} has wing disc phenotype

(Haerry et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Over-activation of the BMP pathway, by co-expressing tkv^{Q199D.Scer\UAS.T:Ivir\HA1} and sax^{Q263D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, leads to an increase of early somatic cells in the male gonad, but no increase in somatic cell proliferation.

(Smendziuk et al., 2015)

Co-expression of tkv^{Q253D.Scer\UAS.cNb} and sax^{Q263D.Scer\UAS.T:Ivir\HA1} in Pdf-positive neurons, under the control of Scer\GAL4^P2.4.Pdf results in a long period phenotype.

(Beckwith et al., 2013)

Introducing one copy of fuss^Scer\UAS.C almost completely rescues the sax^{Q263D.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^A9 wing phenotype - vein formation is close to wild type.

(Fischer et al., 2012)

Pan-neuronal expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} reduces the incidence of defasciculation from 64% to 30% in frac^Δ1 mutants.

Co-expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} partially suppresses the motor axon mis-projections and hypo-fasciculation found upon expression of frac^Scer\UAS.cMa (from 55% to 27%, when both transgenes are expressed under the control of either or both Scer\GAL4^elav-C155 and Scer\GAL4^how-24B).

(Miller et al., 2011)

Expression of Mad^{SDVD.Scer\UAS} in animals expressing sax^{Q263D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 does not enhance the wing vein patterning defects.

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in animals expressing tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 enhances the wing phenotype.

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in animals expressing tkv^{1-A-T318D.Scer\UAS} under the control of Scer\GAL4^A9 enhances the wing phenotype.

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in animals expressing tkv^{1-A-T485D.Scer\UAS} under the control of Scer\GAL4^A9 slightly enhances the wing phenotype.

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in animals expressing tkv^{1-A-T318D-T485D.Scer\UAS} under the control of Scer\GAL4^A9 results in a wing phenotype similar to co-expression with the single mutant tkv^{1-A-T485D.Scer\UAS}.

(Haerry, 2010)

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in motor neurons (under the control of Scer\GAL4^OK6) rescues bouton number and EJP phenotypes in Ranbp11⁷⁰ mutants, although mEJP frequency is not rescued.

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in muscle (under the control of Scer\GAL4^G14) does not rescue bouton number or mEJP and EJP phenotypes in Ranbp11⁷⁰ mutants.

(Higashi-Kovtun et al., 2010)

Overexpression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} enhances the adult visual system degeneration seen in Scer\GAL4^GMR.PF, Ppt1^Scer\UAS.cKa flies.

(Saja et al., 2010)

The wing patterning phenotypes resulting from the expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} with Scer\GAL4^ptc-559.1 are enhanced by the co-expression of kek5^{Scer\UAS.T:Avic\GFP}, and there is an additional decrease in wing size.

(Evans et al., 2009)

Flies expressing sax^{Q263D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^A9 and also carrying tkv^{DE-Q199D.tld.T:Ivir\HA1} have blistered, darkly pigmented wings. Wings discs of these animals show overgrowth.

(Haerry et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

604930

w^*; P{UAS-sax.Q263D.T:HA1}1A3

604931

P{UAS-sax.Q263D.T:HA1}3A, y¹ w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

SAXA

(Haerry et al., 1998)

sax^{Q263D.Scer\UAS.T:Ivir\HA1}

sax^{Q263D.UAS.T:HA1}

sax^{Q263D.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (23)

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