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General Information
Symbol
Dmel\Sp1dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0155796
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Sp1i
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of a 813 fragment of the Sp1 gene inserted as an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Sp1dsRNA.Scer\UAS under the control of Scer\GAL4ap.PU results in the absence of the joint between tarsal segments 4 and 5, with consequent segment fusion.

No defects in dendrite pruning of ddaC dorsal dendritic arborising neurons are seen 18 hours after puparium formation in animals expressing Sp1dsRNA.Scer\UAS under the control of Scer\GAL4ppk.1.9.

Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under the control of Scer\GAL4dac-p7d23 (restricted to second instar stage onwards using Scer\GAL80ts.αTub84B) results in a strong reduction in the size of the medial part of the leg.

Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under the control of Scer\GAL4Dll-em212 (restricted to second instar stage onwards using Scer\GAL80ts.αTub84B) results in a strong reduction in the size of the distal part of the leg.

Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under control of Scer\GAL4btd-MD808 (restricted to second instar stage onwards using Scer\GAL80ts.αTub84B) results in loss of the antennal a1 and a2 segments.

Co-expression of Sp1dsRNA.Scer\UAS, Sp1GD4097 and Dcr-2Scer\UAS.cDa under control of Scer\GAL4btd-MD808 from embryogenesis onwards results in a reduction in the size of the entire leg, from coxa to the distal tip.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

When Sp1dsRNA.Scer\UAS is expressed in a btdMD808 background (driven by the Scer\GAL4btd-MD808 aspect of the P{GawB}btdMD808 insertion), at most some reduction in the tarsus is seen in some mutant legs. When both btddsRNA.Scer\UAS and Sp1dsRNA.Scer\UAS are expressed in a btdMD808 background (at 29oC) and driven by the Scer\GAL4btd-MD808 aspect of the P{GawB}btdMD808 insertion), the animals are unable to hatch and the antennae and legs are affected in all cases, though to different extents. Segments I and II of the tarsus are often reduced. Leg segments are reduced in size and often fused. When these flies are grown at 17oC almost no alteration is seen.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Sp1Scer\UAS.dsRNA
Sp1dsRNA.Scer\UAS
Sp1dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)